Home LiteratureArticle Details
PMID: 507191 Published · ppublish English Journal Article

Chemically induced bidirectional differentiation of embryonal carcinoma cells in vitro.

The American journal of pathology ·Vol. 97 ·No. 3 ·1979-12-00 ·Pages 563-84

Speers WC, Birdwell CR, Dixon FJ

Abstract

N,N-dimethylacetamide, hexamethylene bisacetamide, and Polybrene induced rapid and extensive differentiation in vitro in an otherwise slowly differentiating subline of embryonal carcinoma cells. The type of differentiated cell induced was dependent on the spatial organization of the stem cells during drug treatment. In monalayer culture "epithelial" cells were produced exclusively. However, treatment of aggregated suspension cultures yielded predominantly "fibroblast-like" cells. The undifferentiated embryonal carcinoma cells and the two differentiated cell types were morphologically distinct when examined by light microscopy, scanning electron microscopy, and transmission electron microscopy; and they had differences in cell surface antigens. Both differential cell types produced large amounts of fibronectin, whereas the embryonal carcinoma cells produced only minimal amounts. This system provides a convenient way to induce relatively synchronous differentiation of embryonal carcinoma cells into specific differentiated cell types.

MeSH Terms
Acetamides/pharmacology Antigens, Surface/analysis Cell Differentiation Cell Line Cell Membrane/ultrastructure Cytoplasm/ultrastructure Diamines Epithelial Cells Fibroblasts/cytology Fibronectins/biosynthesis Hexadimethrine Bromide/pharmacology Organoids/ultrastructure Teratoma/pathology
Chemicals
Acetamides Antigens, Surface Diamines Fibronectins Hexadimethrine Bromide dimethylacetamide hexamethylene bisacetamide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Speers W C
Birdwell C R
Dixon F J
References (37)
37 references, click to expand
  1. MULTIPOTENTIALITY OF SINGLE EMBRYONAL CARCINOMA CELLS.
    Cancer Res. 1964 Oct;24:1544-51 PMID: 14234000
  2. Microscopic volume determination and probability.
    Lab Invest. 1963 Apr;12:460-4 PMID: 13953770
  3. Junctional complexes in various epithelia.
    J Cell Biol. 1963 May;17:375-412 PMID: 13944428
  4. Effects of fibroblast and epidermal growth factors on ovarian cell proliferation in vitro. I. Characterization of the response of granulosa cells to FGF and EGF.
    Endocrinology. 1977 Apr;100(4):1108-20 PMID: 300054
  5. The induction of differentiation in teratocarcinoma stem cells by retinoic acid.
    Cell. 1978 Oct;15(2):393-403 PMID: 214238
  6. [Effects of hexamethylenebisacetamide on the differentiation of embryonal carcinoma cells].
    C R Acad Sci Hebd Seances Acad Sci D. 1978 Jan;286(1):109-11 PMID: 206377
  7. Appearance of fibronectin during differentiation of mouse teratocarcinoma in vitro.
    Nature. 1978 Mar 23;272(5651):355-6 PMID: 345125
  8. Expression of a high molecular weight cell surface glycoprotein (LETS protein) by preimplantation mouse embryos and teratocarcinoma stem cells.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2324-8 PMID: 276875
  9. Surface antigen in early differentiation.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4449-52 PMID: 270688
  10. Morphological and biochemical observations on cells derived from the in vitro differentiation of the embryonal carcinoma cell line PCC4-F.
    Cancer Res. 1977 Jul;37(7 Pt 1):2072-81 PMID: 558822
  11. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells.
    Dev Biol. 1977 Dec;61(2):230-44 PMID: 590624
  12. Binding of soluble form of fibroblast surface protein, fibronectin, to collagen.
    Int J Cancer. 1977 Jul 15;20(1):1-5 PMID: 903179
  13. Clonal lines of teratocarcinoma cells in vitro: differentiation and cytogenetic characteristics.
    J Cell Physiol. 1976 NOV;89(3):441-55 PMID: 988033
  14. Plasminogen activator in early embryogenesis: enzyme production by trophoblast and parietal endoderm.
    Cell. 1976 Oct;9(2):231-40 PMID: 975245
  15. Changes in expression of fibroblast surface antigen (SFA) during cytodifferentiation and heterokaryon formation.
    Differentiation. 1976 Jun 4;5(2-3):85-9 PMID: 964519
  16. Cell determination and biochemical differentiation of the early mammalian embryo.
    Curr Top Dev Biol. 1974;8:151-78 PMID: 4596577
  17. Current concepts. cell junctions.
    N Engl J Med. 1972 Mar 9;286(10):521-4 PMID: 4550971
  18. Totipotency and normal differentiation of single teratocarcinoma cells cloned by injection into blastocysts.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):549-53 PMID: 1061157
  19. Fate of teratocarcinoma cells injected into early mouse embryos.
    Nature. 1975 Nov 6;258(5530):70-73 PMID: 1186881
  20. The effect of cells transferred into the mouse blastocyst on subsequent development.
    J Exp Med. 1974 Oct 1;140(4):1049-56 PMID: 4610074
  21. [Teratocarcinoma of the mouse: isolation, culture and properties of pluripotential cells].
    Ann Microbiol (Paris). 1973 Oct;124(3):269-82 PMID: 4599372
  22. Increased susceptibility of murine teratocarcinoma cells to Simian virus 40 and polyoma virus following treatment with 5-bromodeoxyuridine.
    J Cell Physiol. 1976 July;88(3):297-305 PMID: 178674
  23. Induction of erythroid differentiation in murine virus infected eythroleukemia cells by highly polar compounds.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1003-6 PMID: 165480
  24. Neoplastic differentiation: characteristics of cell lines derived from a murine teratocarcinoma.
    J Cell Physiol. 1974 Aug;84(1):13-27 PMID: 4858519
  25. Replication of Sindbis virus. IV. Electron microscope study of the insertion of viral glycoproteins into the surface of infected chick cells.
    J Virol. 1974 Aug;14(2):366-74 PMID: 4858787
  26. Quantitative haemagglutination. 6. Relationship of sialic acid content of red cells and aggregation by Polybrene, protamine and poly-L-lysine.
    Br J Haematol. 1973 Aug;25(2):227-37 PMID: 4726903
  27. The isolation and properties of a clonal tissue culture strain of pluripotent mouse teratoma cells.
    J Embryol Exp Morphol. 1972 Aug;28(1):163-76 PMID: 4672577
  28. In vitro growth and differetiation of clonal populations of multipotential mouse clls derived from a transplantable testicular teratocarcinoma.
    J Natl Cancer Inst. 1970 May;44(5):1001-14 PMID: 4936569
  29. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide.
    Proc Natl Acad Sci U S A. 1971 Feb;68(2):378-82 PMID: 5277089
  30. [Mouse teratocarcinoma: differentiation in cultures of a multipotential primitive cell line (author's transl)].
    Ann Microbiol (Paris). 1975 Jan;126(1):3-22 PMID: 1103673
  31. Immunosurgery of mouse blastocyst.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):5099-102 PMID: 1108013
  32. In vitro astrocytic differentiation from embryoid bodies of an experimental mouse testicular teratoma.
    Am J Pathol. 1976 Apr;83(1):197-212 PMID: 776002
  33. A new group of potent inducers of differentiation in murine erythroleukemia cells.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):862-6 PMID: 1062798
  34. Normal genetically mosaic mice produced from malignant teratocarcinoma cells.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3585-9 PMID: 1059147
  35. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1441-5 PMID: 1055416
  36. Investigation of the determinative state of the mouse inner cell mass. II. The fate of isolated inner cell masses transferred to the oviduct.
    J Embryol Exp Morphol. 1975 Jul;33(4):991-1001 PMID: 1176885
  37. Developmental potentialities of clonal in vitro cultures of mouse testicular teratoma.
    J Natl Cancer Inst. 1970 May;44(5):1015-36 PMID: 5514468
Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1979-12-00
Pages
563-84
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC2042432
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2