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PMID: 5073740 Published · ppublish English Journal Article

The amino acid sequence of the B-protein of bacteriophage ZJ-2.

The Biochemical journal ·Vol. 127 ·No. 1 ·1972-03-00 ·Pages 167-78

Snell DT, Offord RE

Abstract

1. Bacteriophage ZJ-2 is a filamentous male-specific coliphage. Methods are given for its preparation and separation into nucleic acid and the major coat-protein component (B-protein). 2. The determination of the amino acid sequence of the B-protein of bacteriophage ZJ-2 is described. The principal features of this aspect of the work were the special procedures dictated by the insolubility of the protein and the extreme insolubility of its fragments. The sequence is compared with that obtained by other workers for the coat protein of the related bacteriophage fd.

MeSH Terms
Acylation Amino Acid Sequence Chymotrypsin Coliphages/analysis Cyanogen Bromide Electrophoresis Nucleic Acids/analysis Peptides/isolation & purification Sodium Dodecyl Sulfate Succinates Thermolysin Trypsin Viral Proteins/analysis
Chemicals
Nucleic Acids Peptides Succinates Viral Proteins Sodium Dodecyl Sulfate Chymotrypsin Trypsin Thermolysin Cyanogen Bromide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Snell D T
Offord R E
References (21)
21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1972-03-00
Pages
167-78
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1178571
Subset
IM
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