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PMID: 5075260 Published · ppublish English Journal Article

Chemical characterization of the proteins and glycoproteins of mouse liver plasma membranes solubilized by sequential extraction with aqueous and organic solvents.

The Biochemical journal ·Vol. 126 ·No. 3 ·1972-02-00 ·Pages 459-66

Gurd JW, Evans WH, Perkins HR

Abstract

1. A procedure for the stepwise fractionation of the proteins of mouse liver plasma membranes is described. 2. Of the membrane protein 20-25% was soluble in 50mm-sodium carbonate-bicarbonate buffer (pH9.7). This fraction contained a large number of proteins but only 1 major glycoprotein. It was low in sialic acid, amino sugars and phospholipid. 3. Extraction of the alkali-insoluble residue with aq. 33% pyridine solubilized an additional 30-35% of the membrane protein. The pyridine-soluble membrane components were enriched in sialic acid and glucosamine and it was shown that this procedure resulted in the selective extraction of glycoproteins. 4. Gel filtration in sodium dodecyl sulphate resolved the pyridine-soluble proteins into five fractions of decreasing molecular weight and an inverse relationship between molecular weight and sialic acid content was indicated.

MeSH Terms
Acrylates Amino Acids/analysis Animals Bicarbonates Buffers Carbonates Cell Membrane/analysis Chromatography, Gel Electrophoresis Glucosamine/analysis Glycoproteins/analysis,isolation & purification Hydrogen-Ion Concentration Liver/analysis Membrane Potentials Methods Mice Molecular Weight Neuraminic Acids/analysis Phospholipids/analysis Proteins/analysis,isolation & purification
Chemicals
Acrylates Amino Acids Bicarbonates Buffers Carbonates Glycoproteins Neuraminic Acids Phospholipids Proteins Glucosamine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gurd J W
Evans W H
Perkins H R
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47 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1260459
Published
1972-02-00
Pages
459-66
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1178400
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1260459 PMC Full Text View journal details
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