The specific assay of arylsulphatase C, a rat liver microsomal marker enzyme.

The Biochemical journal ·Vol. 128 ·No. 2 ·1972-06-00 ·Pages 331-6

Milson DW, Rose FA, Dodgson KS

Abstract

Conditions based on previous assays with potassium p-acetylphenyl sulphate have been established for the specific assay of arylsulphatase C in rat tissues. The enzyme has optimum activity with 40mm substrate at pH8.0 in the presence of 0.1m-phosphate buffer. Under these conditions arylsulphatase C can be assayed without interference from the other arylsulphatase enzymes present and is useful as a marker for the endoplasmic reticulum in cell-fractionation studies.

MeSH Terms

Animals Buffers Cell Fractionation Endoplasmic Reticulum/enzymology Hydrogen-Ion Concentration In Vitro Techniques Male Microsomes, Liver/enzymology Phosphates Rats Sulfatases/analysis Sulfates

Chemicals

Buffers Phosphates Sulfates Sulfatases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Milson D W

Rose F A

Dodgson K S

References (9)

9 references, click to expand

The sulphatase of ox liver. I. The complex nature of the enzyme.
Biochem J. 1953 Jan;53(1):12-5 PMID: 13032023
Studies on sulphatases. I. The choice of substrate for the assay of rat-liver arylsulphatase.
Biochem J. 1953 Feb;53(3):444-51 PMID: 13032092
Studies on sulphatases. II. The assay of the arylsulphatase activity of rat tissues.
Biochem J. 1953 Feb;53(3):452-7 PMID: 13032093
Studies on sulphatases. 6. The localization of arylsulphatase in the rat-liver cell.
Biochem J. 1954 Feb;56(2):177-81 PMID: 13140169
Studies on sulphatases. IX. The arylsulphatases of mammalian liver.
Biochem J. 1955 Jan;59(1):29-37 PMID: 14351136
The impure nature of nitrocatechol sulphate.
Biochim Biophys Acta. 1956 Jul;21(1):175 PMID: 13363879
Assay of sulfatases.
Methods Biochem Anal. 1957;4:211-55 PMID: 13430367
Studies on sulphatases. 16. A soluble preparation of arysulphatase C of rat-liver microsomes.
Biochem J. 1957 Jun;66(2):357-63 PMID: 13445698
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.
Biochem J. 1955 Aug;60(4):604-17 PMID: 13249955

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1280331

Published

1972-06-00

Pages

331-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1173768

Subset

External Links

PubMed source DOI: 10.1042/bj1280331 PMC Full Text View journal details

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The specific assay of arylsulphatase C, a rat liver microsomal marker enzyme.

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