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PMID: 5103274 Published · ppublish English Journal Article

Deoxyribonucleic acid synthesis in ultraviolet-light-irradiated Chinese hamster cells.

Biophysical journal ·Vol. 11 ·No. 3 ·1971-03-00 ·Pages 295-301

Meyn RE, Humphrey RM

Abstract

Two mammalian cell lines, Chinese hamster ovary (CHO) which can recover colony-forming ability between fractionated doses of ultraviolet light (UV), and Chinese hamster B-14FAF28 which cannot recover, were tested for the ability to bypass UV-induced photoproducts in DNA during postirradiation DNA synthesis. The molecular weight distributions of newly synthesized DNA in UV-irradiated populations of both cell lines showed evidence for photoproduct bypass. Hence, the bypass mechanism does not correlate with recovery after UV.

MeSH Terms
Animals Cell Line Centrifugation, Density Gradient Cricetinae DNA/biosynthesis Female Molecular Weight Ovary/metabolism,radiation effects Radiation Effects Thymidine/metabolism Time Factors Tritium Ultraviolet Rays
Chemicals
Tritium DNA Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Meyn R E
Humphrey R M
References (18)
18 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(71)86215-X
Published
1971-03-00
Pages
295-301
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1483991
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(71)86215-X PMC Full Text View journal details
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