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PMID: 5112205 Published · ppublish English Journal Article

Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. 3. Assembly of the three subclasses of IgG.

The Journal of experimental medicine ·Vol. 134 ·No. 5 ·1971-11-01 ·Pages 1316-34

Baumal R, Potter M, Scharff MD

Abstract

The synthesis, assembly, and secretion of the three major subclasses of mouse IgG has been examined in 14 myeloma tumors and two cultured cell lines as well as in the cells from the popliteal lymph nodes of immunized mice. The total amount of IgG synthesized was between 15 and 43% of the cytoplasmic proteins made during a 15 min period. H(2) and H(2)L were the major precursors of IgG(2a) and IgG(1) but, in all of the tumors, HL was also an intermediate. In contrast, HL was a major precursor of IgG(2b). Most of the noncovalent and covalent assembly of IgG occurred after release of the newly synthesized H and L chains from the polyribosomes and assembly was not completed until 10 min or more after the synthesis of the polypeptide chains.

MeSH Terms
Animals Antibody Formation Carbon Isotopes Cell Line/metabolism Immunoglobulin G/biosynthesis,classification,metabolism Immunoglobulins/biosynthesis,classification,metabolism Kinetics Leucine/metabolism Lymph Nodes/immunology,metabolism Mice Multiple Myeloma/immunology,metabolism Peptides/metabolism Ribosomes/metabolism Threonine/metabolism Valine/metabolism
Chemicals
Carbon Isotopes Immunoglobulin G Immunoglobulins Peptides Threonine Leucine Valine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Baumal R
Potter M
Scharff M D
References (27)
27 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.134.5.1316
Published
1971-11-01
Pages
1316-34
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139014
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.134.5.1316 PMC Full Text View journal details
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