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PMID: 5118106 Published · ppublish English Journal Article

The purification and properties of microsomal palmitoyl-coenzyme A synthetase.

The Biochemical journal ·Vol. 122 ·No. 3 ·1971-04-00 ·Pages 353-62

Bar-Tana J, Rose G, Shapiro B

Abstract

The isolation and purification of palmitoyl-CoA synthetase from rat liver microsomes is described. Several methods suitable for enzyme assay are described. The general properties and kinetic parameters of the purified enzyme were determined and are discussed in relationship to microsomal fatty acid activation.

MeSH Terms
Albumins Ammonium Sulfate Animals Bile Acids and Salts Carbon Isotopes Chromatography Chromatography, Gel Coenzyme A Electrophoresis, Disc Hydroxyapatites Ligases/isolation & purification Microsomes, Liver/enzymology Palmitic Acids Phosphatidylcholines Rats Surface-Active Agents Ultracentrifugation
Chemicals
Albumins Bile Acids and Salts Carbon Isotopes Hydroxyapatites Palmitic Acids Phosphatidylcholines Surface-Active Agents Ligases Coenzyme A Ammonium Sulfate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bar-Tana J
Rose G
Shapiro B
References (28)
28 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1220353
Published
1971-04-00
Pages
353-62
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1176785
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1220353 PMC Full Text View journal details
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