Home LiteratureArticle Details
PMID: 5129252 Published · ppublish English Journal Article

Rabbit Tamm-Horsfall urinary glycoprotein. Chemical composition and subunit structure.

The Biochemical journal ·Vol. 122 ·No. 5 ·1971-05-00 ·Pages 623-31

Marr AM, Neuberger A, Ratcliffe WA

Abstract

1. Tamm-Horsfall glycoprotein from rabbit urine has been isolated and characterized. The homogeneity of the preparation has been established by a variety of procedures including disc gel electrophoresis and ultracentrifugation in aqueous solution, sodium dodecyl sulphate and formic acid. 2. The chemical composition has been determined and a carbohydrate content of approx. 31% was obtained. The relative contents of the amino acids were shown to be very similar to those in human Tamm-Horsfall glycoprotein. A trace of lipid was also detected. 3. Leucine was identified as the only N-terminal amino acid. 4. The subunit structure was investigated in the presence of sodium dodecyl sulphate by gel filtration and disc gel electrophoresis. These studies indicated that the subunit possessed a molecular weight of approx. 84000+/-6000. A similar value was obtained after reduction and S-alkylation of the glycoprotein indicating that the disulphide bonds were all intrachain. 5. A minimum value for the chemical molecular weight of 85000+/-6000 was obtained from the number of N-terminal amino acids released by cyanogen bromide cleavage of the glycoprotein. 6. The immunological properties of the glycoprotein were studied. Cross reactivity was demonstrated between human Tamm-Horsfall glycoprotein and a guinea-pig anti-rabbit Tamm-Horsfall antiserum.

MeSH Terms
Alkylation Amino Acids/analysis Animals Carbohydrates/analysis Chromatography, Gel Cross Reactions Electrophoresis, Disc Gels Glycoproteins/analysis,urine Guinea Pigs Humans Immune Sera Lipids/analysis Molecular Weight Oxidation-Reduction Rabbits Surface-Active Agents Ultracentrifugation
Chemicals
Amino Acids Carbohydrates Gels Glycoproteins Immune Sera Lipids Surface-Active Agents
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Marr A M
Neuberger A
Ratcliffe W A
References (29)
29 references, click to expand
  1. Factors determining the aggregation of urinary mucoprotein.
    J Clin Pathol. 1966 Jul;19(4):392-6 PMID: 5929342
  2. Studies on the electron transfer system. LXVII. Polyacrylamide gel electrophoresis of the mitochondrial electron transfer complexes.
    Arch Biochem Biophys. 1966 Apr;114(1):223-30 PMID: 4289034
  3. The disaggregation of Tamm Horsfall mucroprotein by acetic acid.
    Biochim Biophys Acta. 1966 Jun 29;121(2):292-7 PMID: 4960255
  4. Immunofluorescent localization of Tamm-Horsfall mucoprotein.
    Experientia. 1966 Sep 15;22(9):624-5 PMID: 5339320
  5. Spectroscopic determination of tryptophan and tyrosine in proteins.
    Biochemistry. 1967 Jul;6(7):1948-54 PMID: 6049437
  6. On the composition and structural organization of complex 3 of the mitochondrial electron transfer chain.
    J Biol Chem. 1967 Nov 10;242(21):4876-87 PMID: 6058933
  7. The separation of glucosamine and galactosamine and their glycitols on an amino acid analyser.
    J Chromatogr. 1968 May 21;35(1):106-7 PMID: 5656414
  8. Immunofluorescent localization of Tamm-Horsfall mucoprotein in human kidney.
    J Clin Pathol. 1969 May;22(3):334-9 PMID: 4891482
  9. Some physical properties of lysyl and arginyl-transfer RNA synthetases of Escherichia coli B.
    Biochim Biophys Acta. 1969 Jul 1;181(2):454-64 PMID: 4893081
  10. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  11. Binding of dodecyl sulfate to proteins at high binding ratios. Possible implications for the state of proteins in biological membranes.
    Proc Natl Acad Sci U S A. 1970 Jul;66(3):1002-7 PMID: 5269225
  12. The gross conformation of protein-sodium dodecyl sulfate complexes.
    J Biol Chem. 1970 Oct 10;245(19):5161-5 PMID: 5528242
  13. Gel chromatography of proteins in denaturing solvents. Comparison between sodium dodecyl sulfate and guanidine hydrochloride as denaturants.
    J Biol Chem. 1970 Oct 10;245(19):5166-8 PMID: 5528243
  14. On the determination of molecular weight of proteins and protein subunits in the presence of 6 M guanidine hydrochloride.
    Biochemistry. 1968 Jan;7(1):261-5 PMID: 4921280
  15. Tamm-Horsfall urinary glycoprotein. The chemical composition.
    Biochem J. 1970 Nov;120(2):417-24 PMID: 5493864
  16. Tamm-Horsfall urinary glycoprotein. The subunit structure.
    Biochem J. 1970 Nov;120(2):425-32 PMID: 5493865
  17. The chemical composition and electron microscopic appearance of a protein derived from urinary casts.
    Biochim Biophys Acta. 1970 Aug 21;214(2):299-308 PMID: 4993505
  18. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  19. Ultraviolet absorption spectra of proteins and amino acids.
    Adv Protein Chem. 1952;7:319-86 PMID: 14933256
  20. The viscometric behaviour of a mucoprotein isolated from human urine.
    Aust J Exp Biol Med Sci. 1953 Jun;31(3):255-65 PMID: 13093546
  21. Ultracentrifugation studies of a urinary mucoprotein which reacts with various viruses.
    J Biol Chem. 1955 Jan;212(1):125-33 PMID: 13233215
  22. Determination of serum glycoproteins.
    Methods Biochem Anal. 1955;2:279-311 PMID: 14393571
  23. The thiobarbituric acid assay of sialic acids.
    J Biol Chem. 1959 Aug;234(8):1971-5 PMID: 13672998
  24. THE SENSITIVITY OF THE NEURAMINOSIDIC LINKAGE IN MUCOSUBSTANCES TOWARDS ACID AND TOWARDS NEURAMINIDASE.
    Biochem J. 1963 Nov;89:380-91 PMID: 14084624
  25. KUMAR KS, COX DJ, WALSH KA, NEURATH H: THE AMINO ACID COMPOSITION OF BOVINE PANCREATIC CARBOXYPEPTIDASE A.
    Biochemistry. 1963 Nov-Dec;2:1468-74 PMID: 14093928
  26. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
    Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
  27. RUMINANT UROLITHIASIS. VII. STUDIES ON THE ORIGIN OF TAMM-HORSFALL URINARY MUCOPROTEIN AND ITS PRESENCE IN OVINE CALCULOUS MATRIX.
    Invest Urol. 1965 Mar;2:453-7 PMID: 14261786
  28. Characterization and separation of an inhibitor of viral hemagglutination present in urine.
    Proc Soc Exp Biol Med. 1950 May;74(1):106-8 PMID: 15430405
  29. LOCALIZATION OF UROMUCID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHNIQUE.
    J Histochem Cytochem. 1965 Mar;13:155-60 PMID: 14335886
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1220623
Published
1971-05-00
Pages
623-31
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1176829
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1220623 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2