Utilization of palmitic acid by Mycobacterium avium.

Infection and immunity ·Vol. 4 ·No. 3 ·1971-09-00 ·Pages 199-204

McCarthy C

Abstract

Mycobacterium avium accumulates (14)C-palmitic acid with saturation kinetics; the process is both temperature dependent and pH sensitive. The fatty acid is incorporated into triglyceride in vivo and the conversion is detectable within 5 min after exposure of the cells to (14)C-palmitic acid. The triglyceride is rapidly utilized because (14)CO(2) evolution from it begins within 30 min after (14)C-palmitic acid accumulation. Data from silicic acid column chromatography of extracts of cultures that have divided many times in medium containing (14)C-palmitic acid indicate that a large proportion of the cell lipid is triglyceride, but the radioactivity is widely dispersed among the other lipids. It is estimated that about 5% of the cell dry weight is triglyceride in a postexponential culture.

MeSH Terms

Carbohydrates/analysis Carbon Dioxide/metabolism Carbon Isotopes Chromatography Chromatography, Gas Chromatography, Thin Layer Culture Media Hydrogen-Ion Concentration Kinetics Lipids/analysis Mycobacterium/analysis,metabolism Palmitic Acids/metabolism Temperature Time Factors Triglycerides/analysis,biosynthesis

Chemicals

Carbohydrates Carbon Isotopes Culture Media Lipids Palmitic Acids Triglycerides Carbon Dioxide

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

McCarthy C

References (13)

13 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.4.3.199-204.1971

Published

1971-09-00

Pages

199-204

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC416288

Subset

External Links

PubMed source DOI: 10.1128/iai.4.3.199-204.1971 PMC Full Text View journal details

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Utilization of palmitic acid by Mycobacterium avium.

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