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PMID: 5158908 Published · ppublish English Journal Article

Biosynthesis of the carbohydrate portions of immunoglobulin M.

The Biochemical journal ·Vol. 125 ·No. 1 ·1971-11-00 ·Pages 235-40

Parkhouse RM, Melchers F

Abstract

Incorporations of radioactive mannose, galactose and fucose into MOPC 104E mouse plasma-cell tumour suspensions suggest a stepwise addition of carbohydrate residues to immunoglobulin M (IgM) during the process of secretion. Mannose and glucosamine residues are added at an early stage, whereas galactose and fucose are added just before, or at the time that, IgM leaves the cell. Free light chains secreted in excess by the same tumour cells incubated with mannose, galactose or fucose contained barely detectable amounts of radioactivity.

MeSH Terms
Animals Antigen-Antibody Complex Carbonates/biosynthesis Chemical Precipitation Chromatography Electrophoresis, Disc Fucose/metabolism Galactose/metabolism Hydrolysis Immune Sera Immunoglobulins/biosynthesis In Vitro Techniques Leucine/metabolism Mannose/metabolism Mice Neoplasms, Experimental/metabolism Plasmacytoma/metabolism Rabbits Time Factors Tritium
Chemicals
Antigen-Antibody Complex Carbonates Immune Sera Immunoglobulins Tritium Fucose Leucine Mannose Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Parkhouse R M
Melchers F
References (18)
18 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1250235
Published
1971-11-00
Pages
235-40
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1178045
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1250235 PMC Full Text View journal details
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