Amino acid sequences around the cysteine residues of rabbit muscle triose phosphate isomerase.

The Biochemical journal ·Vol. 122 ·No. 2 ·1971-04-00 ·Pages 209-18

Miller JC, Waley SG

Abstract

1. The nature of the subunits in rabbit muscle triose phosphate isomerase has been investigated. 2. Amino acid analyses show that there are five cysteine residues and two methionine residues/subunit. 3. The amino acid sequences around the cysteine residues have been determined; these account for about 75 residues. 4. Cleavage at the methionine residues with cyanogen bromide gave three fragments. 5. These results show that the subunits correspond to polypeptide chains, containing about 230 amino acid residues. The chains in triose phosphate isomerase seem to be shorter than those of other glycolytic enzymes.

MeSH Terms

Amino Acid Sequence Amino Acids/analysis Animals Chromatography, Ion Exchange Chromatography, Paper Cyanogen Bromide Cysteine/analysis Electrophoresis Glycerophosphates Isomerases/analysis Methionine/analysis Muscles/enzymology Peptide Hydrolases Peptides/analysis Rabbits

Chemicals

Amino Acids Glycerophosphates Peptides Methionine Peptide Hydrolases Isomerases Cysteine Cyanogen Bromide

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Miller J C

Waley S G

References (29)

29 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1220209

Published

1971-04-00

Pages

209-18

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1176764

Subset

External Links

PubMed source DOI: 10.1042/bj1220209 PMC Full Text View journal details

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Amino acid sequences around the cysteine residues of rabbit muscle triose phosphate isomerase.

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