Indirect hemagglutination with Mycoplasma antigens: effects of pH on antigen sensitization of tanned fresh and formalinized sheep erythrocytes.

Applied microbiology ·Vol. 22 ·No. 5 ·1971-11-00 ·Pages 756-9

Krogsgaard-Jensen A

Abstract

An investigation of the influence of different factors affecting the sensitivity of the indirect hemagglutination test has been performed with antigens of four mycoplasmas isolated from sheep or goat. Tanned erythrocytes of sheep, fresh and formalinized, were sensitized with the above antigens. It was demonstrated that, with formalinized erythrocytes, the sensitivity was increased by 50 to 100 times when the sensitization was done at a low pH level. The pH level was unimportant for sensitizing fresh erythrocytes. The greatest sensitivity of the indirect hemagglutination test was obtained with fresh rather than formalinized erythrocytes. Three different types of antigens were used, and the most suitable antigen was found to be the supernatant fluid from an ultrasonically treated centrifuged Mycoplasma suspension.

MeSH Terms

Animals Antigens Antigens, Bacterial Cross Reactions Erythrocytes/immunology Formaldehyde Goats Hemagglutination Tests/standards Hydrogen-Ion Concentration Immune Sera Methods Mycoplasma/immunology Rabbits Sheep Species Specificity Tannins

Chemicals

Antigens Antigens, Bacterial Immune Sera Tannins Formaldehyde

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Krogsgaard-Jensen A

References (9)

9 references, click to expand

Use of formalinized Mycoplasma gallisepticum antigens and chicken erythrocytes in hemagglutination and hemagglutination-inhibition studies.
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A study of factors influencing the sensitivity to antibody of tanned, ovalbumin-coated, formalinized sheep red cells.
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An indirect haemagglutination test for serum antibodies against mycoplasma pneumoniae using formalinized, tanned sheep erythrocytes.
Acta Pathol Microbiol Scand. 1968;73(3):459-72 PMID: 4878744
Effect of pH on the immunogenicity of Mycoplasma pneumoniae.
J Bacteriol. 1969 Feb;97(2):612-9 PMID: 4975150
HEMAGGLUTINATION STUDIES WITH FORMALINIZED ERYTHROCYTES. EFFECT OF BIS-DIAZO-BENZIDINE AND TANNIC ACID TREATMENT ON SENSITIZATION BY SOLUBLE ANTIGEN.
J Immunol. 1963 May;90:663-71 PMID: 14053285
Comparison of isolates of Mycoplasma hyorhinis by indirect hemagglutination.
Am J Vet Res. 1963 May;24:622-7 PMID: 13975123
AN INDIRECT HEMAGGLUTINATION TEST FOR DIAGNOSIS OF MYCOPLASMA PNEUMONIAE INFECTIONS.
Proc Soc Exp Biol Med. 1964 Aug-Sep;116:947-50 PMID: 14230396
SIGNIFICANCE OF ANTIBODY TO MYCOPLASMA HOMINIS TYPE 1 AS MEASURED BY INDIRECT HEMAGGLUTINATION.
Proc Soc Exp Biol Med. 1965 Apr;118:1073-83 PMID: 14277667
Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells.
J Immunol. 1954 May;72(5):360-7 PMID: 13163409

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.22.5.756-759.1971

Published

1971-11-00

Pages

756-9

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC376412

Subset

External Links

PubMed source DOI: 10.1128/am.22.5.756-759.1971 PMC Full Text

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Indirect hemagglutination with Mycoplasma antigens: effects of pH on antigen sensitization of tanned fresh and formalinized sheep erythrocytes.

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