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PMID: 521457 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Ultrastructure of double minutes from a human tumor cell line.

The Journal of cell biology ·Vol. 83 ·No. 3 ·1979-12-00 ·Pages 663-6

Barker PE, Stubblefield E

Abstract

Double minutes (dm) have been isolated from human tumor cells by zonal centrifugation and by differential pelleting of chromosome suspsension. These methods allowed collection of dm in sufficient quantity and purity for visualization with electron microscopy. Ultrastructurally, the chromatin fibers in dm resemble thrance fragments was found. When the two isolation protocols were compared, differential pelleting was shown to increase purity twofold to 85% dm by mass. The differential pelleting procedure enables easy collection of dm in sufficient quantity and purity for chemical analysis.

MeSH Terms

Carcinoma Cell Cycle Cell Fractionation Cell Line Cell Nucleus/ultrastructure Centrifugation, Zonal Chromatin/ultrastructure Chromosomes/ultrastructure Humans Metaphase

Chemicals

Chromatin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Barker P E

Stubblefield E

References (15)

15 references, click to expand

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Double minute chromosomes and the homogeneously staining regions in chromosomes of a human neuroblastoma cell line.
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Chicken macrochromosomes contain an endogenous provirus and microchromosomes contain sequences related to the transforming gene of ASV.
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Double minutes in human carcinoma cell lines, with special reference to breast tumors.
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Specific measurement of DNA in nuclei and nucleic acids using diaminobenzoic acid.
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Experimental elimination and recovery of double minute chromosomes in malignant cell populations.
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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.83.3.663

Published

1979-12-00

Pages

663-6

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2110517

Subset

External Links

PubMed source DOI: 10.1083/jcb.83.3.663 PMC Full Text View journal details

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