Biosynthesis of insulin in bovine fetal pancreatic slices: the incorporation of tritiated leucine into a single-chain proinsulin, a double-chain intermediate, and insulin in subcellular fractions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 63 ·No. 2 ·1969-06-00 ·Pages 442-9

Tung AK, Yip CC

Abstract

This communication reports the biosynthesis of insulin in the bovine fetal pancreatic slices in vitro. Double-chain proinsulin and insulin were found as major components in the mitochondrial-granule fraction of bovine fetal pancreas. Tritiated leucine was incorporated into a single-chain proinsulin, a double-chain proinsulin, and insulin. Subcellular fractionation of the slices incubated with tritiated leucine showed that radioactive single-chain proinsulin was present in the deoxycholate-soluble microsomal fraction, deoxycholate-insoluble microsomal fraction, and mitochondrial-granule fraction. Labeled double-chain proinsulin and insulin were present in the deoxycholate-soluble microsomal and mitochondrial-granule fractions. These results are consistent with the hypothesis that insulin is synthesized as a single-chain polypeptide on the ribosomes, and that intracellular proteolysis in the subcellular membranous organelles and beta-granules converts the single-chain proinsulin to insulin via a double-chain intermediate.

MeSH Terms

Animals Cattle Chromatography, Gel Fetus Histocytochemistry Immunoelectrophoresis In Vitro Techniques Insulin/biosynthesis Leucine/metabolism Microscopy, Electron Mitochondria Pancreas/metabolism Tritium

Chemicals

Insulin Tritium Leucine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Tung A K

Yip C C

References (17)

17 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.63.2.442

Published

1969-06-00

Pages

442-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC223584

Subset

External Links

PubMed source DOI: 10.1073/pnas.63.2.442 PMC Full Text View journal details

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Biosynthesis of insulin in bovine fetal pancreatic slices: the incorporation of tritiated leucine into a single-chain proinsulin, a double-chain intermediate, and insulin in subcellular fractions.

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