Abstract
Transfer RNA fractions from Saccharomyces lactis and Saccharomyces cerevisiae have been tested for cytokinin activity in the tobacco bioassay. Cysteine tRNA has been identified as a cytokinin-containing tRNA species in S. cerevisiae. Acid hydrolysates of S. lactis tRNA fractions (containing arginine tryptophan, and valine acceptor activities) and S. cerevisiae tRNA fractions (containing alanine, asparagine, aspartic acid, glutamic acid, glycine, histidine, tryptophan, and valine acceptor activities) were inactive in the tobacco bioassay. Cytokinins have been found only in those tRNA species corresponding to codons beginning with U.
MeSH Terms
Biological Assay
Chromatography
Kinins/analysis
Plant Growth Regulators
RNA, Transfer/analysis
Saccharomyces
Chemicals
Kinins
Plant Growth Regulators
RNA, Transfer
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Armstrong D J
Skoog F
Kirkegaard L H
Hampel A E
Bock R M
Gillam I
Tener G M
References (24)
24 references, click to expand
-
Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine.
Science. 1968 Aug 16;161(3842):691-3
PMID: 4874577
-
2-Methylthio N6-(delta 2-isopentenyl) adenosine: a component of E. coli tyrosine transfer RNA.
Biochem Biophys Res Commun. 1968 Oct 24;33(2):299-306
PMID: 4881052
-
Large oligonucleotides isolated from yeast tyrosine transfer ribonucleic acid after partial digestion with ribonuclease T1.
J Biol Chem. 1967 Mar 25;242(6):1324-30
PMID: 6023574
-
Studies on polynucleotides. LXXIX. Yeast phenylalanine transfer ribonucleic acid: products obtained by degradation with pancreatic ribonuclease.
J Biol Chem. 1968 Feb 10;243(3):575-83
PMID: 5637709
-
The incorporation of mevalonic acid into the N6-(delta 2-isopentenyl) adenosine of transfer ribonucleic acid in Lactobacillus acidophilus.
Biochemistry. 1968 Jan;7(1):472-82
PMID: 4991483
-
STRUCTURE OF A RIBONUCLEIC ACID.
Science. 1965 Mar 19;147(3664):1462-5
PMID: 14263761
-
A cytokinin in soluble RNA from a higher plant.
Life Sci. 1967 Feb 15;6(4):387-94
PMID: 6032211
-
Cytokinins in ribonucleic acids.
Nature. 1966 Sep 3;211(5053):1093-5
PMID: 5970107
-
Incorporation of a kinin, N, 6-benzyladenine into soluble RNA.
Plant Physiol. 1966 Jan;41(1):75-82
PMID: 16656236
-
N-6-(delta-3-isopentenyl) adenosine. A component of the transfer ribonucleic acid of yeast and of mammalian tissue, methods of isolation, and characterization.
Biochemistry. 1967 Jun;6(6):1837-48
PMID: 6035922
-
Cytokinin activity: localization in transfer RNA preparations.
Science. 1966 Dec 9;154(3754):1354-6
PMID: 5332571
-
Characterization of labeled ribonucleic acid from tissue grown on 14C-containing cytokinins.
J Biol Chem. 1967 Oct 10;242(19):4490-4
PMID: 6070845
-
Oligonucleoides from yeast tyrosine transfer ribonucleic acid.
J Biol Chem. 1967 Mar 25;242(6):1318-23
PMID: 6023573
-
A column chromatographic procedure for the fractionation of s-RNA.
Biochemistry. 1965 Jun;4(6):1174-83
PMID: 5840002
-
Cytokinins in the soluble RNA of plant tissues.
Science. 1967 Apr 7;156(3771):69-71
PMID: 6020041
-
A general procedure for the isolation of specific transfer ribonucleic acids.
Biochemistry. 1968 Oct;7(10):3459-68
PMID: 5681458
-
Biosynthesis of N6-(delta-2-isopentenyl) adenosine. The precursor relationship of acetate and mevalonate to the delta-2-isopentenyl group of the transfer ribonucleic acid of microorganisms.
Biochemistry. 1968 Mar;7(3):940-4
PMID: 5657858
-
Studies on polynucleotides, lxviii the primary structure of yeast phenylalanine transfer RNA.
Proc Natl Acad Sci U S A. 1967 Mar;57(3):751-8
PMID: 16591527
-
Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding.
J Mol Biol. 1969 Jan 14;39(1):145-57
PMID: 4938812
-
Development of cytokinin activity by rearrangement of 1-substituted adenines to 6-substituted aminopurines: inactivation by N6, 1-cyclization.
Proc Natl Acad Sci U S A. 1966 Aug;56(2):709-16
PMID: 5229988
-
The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose.
Biochemistry. 1967 Oct;6(10):3043-56
PMID: 6056973
-
, Occolowitz J, Burrows WJ, Armstrong DJ, Skoog F, Bock RM, Gillam I, Tener GM: Cytokinins: isolation identification of 6-(3-methyl-2-butenylamino)-9-beta-D-ribyfuranosylpurine (2iPA) from yeast cysteine tRNA.
Biochem Biophys Res Commun. 1969 Apr 29;35(2):205-9
PMID: 5772580
-
A specific modification next to the anticodon of phenylalanine transfer ribonucleic acid.
Eur J Biochem. 1968 Sep 24;5(4):546-55
PMID: 5698615
-
Cytokinins in tRNA of Corynebacterium fascians.
Plant Physiol. 1968 Mar;43(3):451-3
PMID: 16656786