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PMID: 5259761 Published · ppublish English Journal Article

Transformation and chromosome changes induced by Epstein-Barr virus in normal human leukocyte cultures.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 63 ·No. 3 ·1969-07-00 ·Pages 740-7

Gerper P, Whang-Peng J, Monroe JH

Abstract

Three separate attempts to establish long-term leukocyte cultures from the peripheral blood of a healthy adult who had no evidence of previous infection with Epstein-Barr virus were consistently unsuccessful. On the other hand, in vitro infection of buffy-coat cells of this donor with Epstein-Barr virus resulted in blastoid transformation of the lymphocytes and long-term growth potential. The transformation was accompanied by major chromosome changes. The significance of these findings in relation to lymphoproliferative diseases in man remains to be determined.

MeSH Terms
Cell Transformation, Neoplastic Chromosome Aberrations Culture Techniques Herpesviridae Humans Karyotyping Leukocytes/cytology Microscopy, Electron Viral Interference
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gerper P
Whang-Peng J
Monroe J H
References (27)
27 references, click to expand
  1. Morphologic, cytogenetic and virologic studies in vitro of a malignant lymphoma from an African child.
    Int J Cancer. 1966 Jan;1(1):89-106 PMID: 5902320
  2. Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope.
    Cancer. 1966 Nov;19(11):1725-42 PMID: 5224274
  3. Cloning of immunoglobulin-producing human leukemic and lymphoma cells in long-term cultures.
    Proc Soc Exp Biol Med. 1967 Jan;124(1):107-11 PMID: 4163469
  4. Involvement of C group chromosomes in five Burkitt lymphoma cell lines.
    J Natl Cancer Inst. 1967 Feb;38(2):209-22 PMID: 6019732
  5. Culture of normal human leukocytes.
    JAMA. 1967 Feb 20;199(8):519-24 PMID: 4960081
  6. Herpes-type virus and chromosome marker in normal leukocytes after growth with irradiated Burkitt cells.
    Science. 1967 Sep 1;157(3792):1064-5 PMID: 6036237
  7. Burkitt lymphoma in New Guinea: establishment of a line of lymphoblasts in vitro and description of their fine structure.
    J Natl Cancer Inst. 1967 Nov;39(5):933-45 PMID: 6066443
  8. Establishment of cell lines from peripheral leucocytes in infectious mononucleosis.
    Nature. 1967 Nov 25;216(5117):810-1 PMID: 6074953
  9. Studies on leukocytes growing in continuous culture derived from normal human donors.
    J Natl Cancer Inst. 1968 Apr;40(4):855-66 PMID: 4869137
  10. C-group chromosome marker in long-term leukocyte cultures.
    J Natl Cancer Inst. 1968 Sep;41(3):795-804 PMID: 5692237
  11. Chromosome studies on cultured lymphoblast cell lines from cases of New Guinea Burkitt lymphoma, myeloblastic and lymphoblastic leukaemia and infectious mononucleosis.
    Int J Cancer. 1968 Sep 15;3(5):644-53 PMID: 5248028
  12. Infectious mononucleosis: in vitro evidence for limited lymphoproliferation.
    Blood. 1969 Feb;33(2):292-9 PMID: 4886119
  13. Transformation of foetal human keukocytes in vitro by filtrates of a human leukaemic cell line containing herpes-like virus.
    Int J Cancer. 1968 Nov 15;3(6):857-66 PMID: 4894385
  14. Amino acid requirements of the Novikoff hepatoma in vitro.
    Proc Soc Exp Biol Med. 1959 Jan;100(1):115-8 PMID: 13634054
  15. Application of a microtechnique to viral serological investigations.
    J Immunol. 1962 Mar;88:320-9 PMID: 13910995
  16. Chromosome preparations of bone marrow cells without prior in vitro culture or in vivo colchicine administration.
    Stain Technol. 1962 Jan;37:17-20 PMID: 13921436
  17. Cell transformations and mitoses produced in vitro by tuberculin purified protein derivative in human blood cells.
    Am Rev Respir Dis. 1963 May;87:734-8 PMID: 13987040
  18. CYTOLOGY OF BURKITT'S TUMOUR (AFRICAN LYMPHOMA).
    Lancet. 1964 Feb 1;1(7327):238-40 PMID: 14086209
  19. CULTIVATION IN VITRO OF HUMAN LYMPHOBLASTS FROM BURKITT'S MALIGNANT LYMPHOMA.
    Lancet. 1964 Feb 1;1(7327):252-3 PMID: 14090852
  20. THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES.
    Blood. 1964 Jan;23:108-16 PMID: 14107045
  21. VIRUS PARTICLES IN CULTURED LYMPHOBLASTS FROM BURKITT'S LYMPHOMA.
    Lancet. 1964 Mar 28;1(7335):702-3 PMID: 14107961
  22. MITOGENIC ACTIVITY IN PHYTOLACCA AMERICANA (POKEWEED).
    Lancet. 1964 Nov 21;2(7369):1100-1 PMID: 14207901
  23. MITOGENIC ACTION OF ANTILEUCOCYTE IMMUNE SERUM ON PERIPHERAL LEUCOCYTES IN VITRO.
    Lancet. 1963 Aug 24;2(7304):385-6 PMID: 14044293
  24. Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes.
    Cancer Res. 1960 May;20:462-6 PMID: 14427849
  25. FINE STRUCTURAL ORGANIZATION OF HUMAN LYMPHOBLASTS OF A TISSUE CULTURE STRAIN (EB1) FROM BURKIT'S LYMPHOMA.
    J Natl Cancer Inst. 1965 Feb;34:241-53 PMID: 14293791
  26. BURKITT TUMOR: TISSUE CULTURE, CYTOGENETIC AND VIRUS STUDIES.
    J Natl Cancer Inst. 1965 Feb;34:319-27 PMID: 14293797
  27. A STUDY OF MALIGNANT TUMOURS IN NIGERIA BY SHORT-TERM TISSUE CULTURE.
    J Clin Pathol. 1965 May;18:261-73 PMID: 14304234
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.63.3.740
Published
1969-07-00
Pages
740-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC223514
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.63.3.740 PMC Full Text View journal details
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