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PMID: 5260921 Published · ppublish English Journal Article

Evidence for a proposed initiation complex for protein synthesis in reticulocyte polyribosome profiles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 63 ·No. 4 ·1969-08-00 ·Pages 1206-13

Hoerz W, McCarty KS

Abstract

Polysomes consisting of 40S ribosomal subunits attached to monosomes, disomes, trisomes, and tetrasomes have been isolated for the first time by the use of isokinetic sucrose density gradients. They are formed upon incubation of rabbit reticulocyte lysate in the presence of 10 mM NaF. It is proposed that these structures represent transient initiation complexes for protein synthesis.

MeSH Terms
Animals Cell-Free System Centrifugation, Density Gradient In Vitro Techniques Phosphorus Isotopes Protein Biosynthesis RNA/analysis Rabbits Reticulocytes/metabolism Ribosomes/analysis
Chemicals
Phosphorus Isotopes RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hoerz W
McCarty K S
References (26)
26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.63.4.1206
Published
1969-08-00
Pages
1206-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC223451
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.63.4.1206 PMC Full Text View journal details
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