Molecular hybridization of radioactive DNA to the DNA of cytological preparations.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 2 ·1969-10-00 ·Pages 600-4

Pardue ML, Gall JG

Abstract

A method is presented for detecting the cellular location of specific DNA fractions. The technique involves the hybridization of a radioactive test DNA in solution to the stationary DNA of a cytological preparation. Sites of DNA binding are then detected by autoradiography. Experiments with DNA of the toad Xenopus are described.

MeSH Terms

Animals Autoradiography DNA Female Hybridization, Genetic Methods Mice Ovum Thymidine/metabolism Tritium

Chemicals

Tritium DNA Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Pardue M L

Gall J G

References (17)

17 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.64.2.600

Published

1969-10-00

Pages

600-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC223386

Subset

External Links

PubMed source DOI: 10.1073/pnas.64.2.600 PMC Full Text View journal details

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Molecular hybridization of radioactive DNA to the DNA of cytological preparations.

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