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PMID: 5261037 Published · ppublish English Journal Article

Studies on the proteins of phi X174. II. The protein composition of the phi X coat.

Burgess AB

Abstract

In order to determine the composition of the PhiX174 coat, PhiX particles labeled in most of the amino acids have been dissociated, and the proteins separated by electrophoresis on polyacrylamide gels. The molar ratios of the four proteins calculated from the fraction of radioactivity in each peak and the molecular weights of the individual proteins suggest that the capsid is composed of 60 molecules of the 48,000 daltons protein and that each of the 12 spikes is composed of one molecule of the 36,000 daltons protein and five each of the 19,000 and 5,000 daltons proteins.

MeSH Terms
Amino Acids/analysis Coliphages Electrophoresis Models, Chemical Molecular Weight Tritium Viral Proteins/analysis
Chemicals
Amino Acids Viral Proteins Tritium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Burgess A B
References (12)
12 references, click to expand
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  6. On the origin of the 70 S component of bacteriophage phi-X174.
    Virology. 1969 Jan;37(1):132-4 PMID: 4883123
  7. The process of infection with bacteriophage phi-X174. 28. Removal of the spike proteins from the phage capsid.
    J Mol Biol. 1969 Jun 28;42(3):547-57 PMID: 5804158
  8. Studies on phiX174 proteins. I. Phage-specific proteins synthesized after infection of Escherichia coli.
    J Mol Biol. 1969 Sep 28;44(3):377-86 PMID: 4899472
  9. The structure of bacteriophage phi-X174.
    Virology. 1961 Sep;15:1-7 PMID: 13778169
  10. Studies on bacteriophage phi-X174 and its DNA by electron microscopy.
    J Mol Biol. 1962 Mar;4:173-8 PMID: 14468002
  11. Physical principles in the construction of regular viruses.
    Cold Spring Harb Symp Quant Biol. 1962;27:1-24 PMID: 14019094
  12. THE PHYSICAL PROPERTIES OF A PROTEIN ISOLATED FROM BACTERIOPHAGE PHI-X174.
    J Mol Biol. 1963 Oct;7:388-400 PMID: 14066616
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1969-10-00
Pages
613-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC223388
Subset
IM
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