On the replication of incomplete chromosomes of phage T4.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 2 ·1969-10-00 ·Pages 747-54

Mosig G, Werner R

Abstract

Some small particles of phage T4 contain incomplete chromosomes measuring two thirds of the length of normal T4 chromosomes. They cannot produce progeny phage after single infection because their incomplete chromosomes lack random segments of the genetic map. We have investigated the replication of DNA following single infection with small particles. Our results indicate that approximately two thirds of the incomplete chromosomes can initiate DNA replication and apparently extend the process from a genetically fixed origin to one end of the molecule. However, few if any of the incomplete chromosomes can initiate a second round of replication. The incomplete chromosomes which do not replicate also do not attach to bacterial membranes.

MeSH Terms

Centrifugation, Density Gradient Chromosome Mapping Chromosomes Coliphages DNA Replication DNA, Viral Hybridization, Genetic Phosphorus Isotopes Tritium Virus Replication

Chemicals

DNA, Viral Phosphorus Isotopes Tritium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mosig G

Werner R

References (15)

15 references, click to expand

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Cold Spring Harb Symp Quant Biol. 1968;33:361-9 PMID: 4891976
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Sedimentation rate as a measure of molecular weight of DNA.
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Evidence for long DNA strands in the replicating pool after T4 infection.
Proc Natl Acad Sci U S A. 1968 Jan;59(1):131-8 PMID: 4873341
Sedimentation Coefficient and Fragility under Hydrodynamic Shear as Measures of Molecular Weight of the DNA of Phage T5.
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J Mol Biol. 1968 May 14;33(3):679-92 PMID: 4882615
Studies on the nature of replicating DNA in T4-infected Escherichia coli.
J Mol Biol. 1966 Jun;18(1):127-43 PMID: 5337554
A map of distances along the DNA molecule of phage T4.
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Failure of incomplete T4 genomes to replicate under conditions of single infection.
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Molecular recombination in T4 bacteriophage deoxyribonucleic acid. I. Tertiary structure of early replicative and recombining deoxyribonucleic acid.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.64.2.747

Published

1969-10-00

Pages

747-54

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC223407

Subset

External Links

PubMed source DOI: 10.1073/pnas.64.2.747 PMC Full Text View journal details

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On the replication of incomplete chromosomes of phage T4.

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