The process of infection with bacteriophage phiX174. XXX. Replication of double-stranded phiX DNA.
Intermediates involved in the replication of double-stranded varphiX174 RF DNA have been identified and partially characterized. Analysis of pulselabeled RF DNA suggests that the synthesis of progeny RF molecules involves, in part, the addition of nucleotides to linear complementary strands on a circular parental strand as template, so as to produce intermediate DNA strands of greater than viral length. Electron microscopy reveals DNA rings with "tails" and "double rings," which could be the intermediate structures. A model is postulated for the replication process.
16 references, click to expand
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Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.Proc Natl Acad Sci U S A. 1968 Feb;59(2):598-605 PMID: 4967086
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