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PMID: 52633 Published · ppublish English Journal Article

Uranyl-EDTA-hematoxylin: a new selective staining technique for nucleolar material.

Histochemistry ·Vol. 43 ·No. 4 ·1975-06-09 ·Pages 313-22

Stockert JC

Abstract

This paper deals with the visualization of nuclear structures in glutaraldehyde fixed, acetic acid flattened preparations from Chironomus salivary glands, by means of an uranyl mordanting followed by hematoxylin staining. Under these conditions all the nuclear structures (bands, Balbiani rings, and nucleoli) were deeply stained. Treatment with 0.1 M EDTA for at least 30 sec after uranyl mordanting completely prevents the following hematoxylin staining in all the structures but the nucleolus. With increased EDTA extraction times (60-90 sec) the central region (composed of pars fibrosa) in spontaneously or experimentally segregated nucleoli showed the highest capacity for retaining uranyl ions. This selective staining of the nucleolar (possibly proteinic) material proved also efficient in cells from Drosophila testes and Allium roots.

MeSH Terms
Animals Benzopyrans Cell Nucleolus/ultrastructure Cell Nucleus/ultrastructure Chromosomes/ultrastructure Diptera/cytology Drosophila/cytology Edetic Acid Hematoxylin Histocytochemistry Male Plant Cells Staining and Labeling Uranium
Chemicals
Benzopyrans Uranium Edetic Acid Hematoxylin
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Stockert J C
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26 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00490190
Published
1975-06-09
Pages
313-22
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00490190
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