Isolation of microtubule protein from cultured mouse neuroblastoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 65 ·No. 1 ·1970-01-00 ·Pages 129-36

Olmsted JB, Carlson K, Klebe R, Ruddle F, Rosenbaum J

Abstract

The addition of vinblastine to high-speed supernatants derived from homogenates of cultured mouse neuroblastoma cells results in the formation of a precipitate which has been characterized as microtubule protein by the following criteria: colchicine-binding activity, molecular weight, amino acid composition, and electrophoretic mobility. The method therefore permits the rapid isolation of microtubule protein from crude supernatants of neuroblastoma cells.

MeSH Terms

Amino Acids/analysis Animals Centrifugation Chemical Precipitation Chromatography, Gel Colchicine Culture Techniques Electrophoresis, Disc Eukaryota Flagella Mice Mitosis Molecular Weight Neoplasm Proteins/analysis,isolation & purification Neoplasms, Experimental Nerve Tissue Proteins/analysis,isolation & purification Neuroblastoma Organoids/analysis Protein Binding Proteins Tritium Vinblastine

Chemicals

Amino Acids Neoplasm Proteins Nerve Tissue Proteins Proteins Tritium Vinblastine Colchicine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Olmsted J B

Carlson K

Klebe R

Ruddle F

Rosenbaum J

References (15)

15 references, click to expand

The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein.
J Cell Biol. 1967 Aug;34(2):525-33 PMID: 6068183
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.
Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20 PMID: 4861258
Procedure for embedding in situ selected cells cultured in vitro.
J Cell Biol. 1967 Oct;35(1):279-83 PMID: 4863049
On the structural protein of flagellar outer fibers.
J Mol Biol. 1968 Mar 14;32(2):277-83 PMID: 5689362
The binding of colchicine by sarcoma 180 cells.
Biochem Pharmacol. 1968 Mar;17(3):477-80 PMID: 5661656
Properties of the protein subunit of central-pair and outer-doublet microtubules of sea urchin flagella.
J Cell Biol. 1968 Aug;38(2):304-15 PMID: 5664206
A high-resolution method for slicing and counting radioactivity in polyacrylamide gels.
Anal Biochem. 1968 Jul;24(1):44-53 PMID: 5665199
Effects of mitotic spindle inhibitors on neurotubules and neurofilaments in anterior horn cells.
J Cell Biol. 1968 Jul;38(1):224-9 PMID: 5691976
Neuronal changes induced by intrathecal vincristine sulfate.
J Neuropathol Exp Neurol. 1968 Oct;27(4):645-58 PMID: 5696051
Microtubular crystals in mammalian cells.
J Cell Biol. 1969 Jan;40(1):95-107 PMID: 5782454
The colchicine-binding protein of mammalian brain and its relation to microtubules.
Biochemistry. 1968 Dec;7(12):4466-79 PMID: 5700666
Vinblastine produces uniaxial, birefringent crystals in starfish oocytes.
J Cell Biol. 1969 Aug;42(2):596-9 PMID: 5815503
Induction in vitro of microtubular crystals by vinca alkaloids.
Science. 1969 Aug 1;165(3892):495-6 PMID: 5793242
Vinblastine-induced precipitation of microtubule protein.
Science. 1969 Aug 1;165(3892):498-9 PMID: 5815703
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.65.1.129

Published

1970-01-00

Pages

129-36

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC286201

Subset

External Links

PubMed source DOI: 10.1073/pnas.65.1.129 PMC Full Text View journal details

Analysis Services

Isolation of microtubule protein from cultured mouse neuroblastoma cells.

Contact

WeChat Official Account