Nuclear magnetic resonance spectroscopy: 13C spectra of some common nucleotides.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 65 ·No. 1 ·1970-01-00 ·Pages 19-26

Dorman DE, Roberts JD

Abstract

Natural-abundance (13)C nuclear magnetic resonance spectra of ten common nucleotides in neutral, aqueous solutions have been determined and interpreted. The spectra of two of these substances were also determined in acidic solutions, and several of the carbon chemical shifts were found to depend markedly on pH. Within the limited range of concentrations employed, there were observed no carbon chemical-shift changes which could be ascribed to base-stacking or base-pairing phenomena.

MeSH Terms

Adenine Nucleotides/analysis Adenosine Triphosphate/analysis Carbon Isotopes Cytosine Nucleotides/analysis Guanine Nucleotides/analysis Magnetic Resonance Spectroscopy Nucleotides/analysis Pentoses/analysis Ribose/analysis Solutions Uracil Nucleotides/analysis

Chemicals

Adenine Nucleotides Carbon Isotopes Cytosine Nucleotides Guanine Nucleotides Nucleotides Pentoses Solutions Uracil Nucleotides Ribose Adenosine Triphosphate

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dorman D E

Roberts J D

References (5)

5 references, click to expand

Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid.
Nature. 1953 Apr 25;171(4356):737-8 PMID: 13054692
Carbon-13 nuclear magnetic resonance: naturally occurring nucleosides.
Proc Natl Acad Sci U S A. 1970 Jan;65(1):27-30 PMID: 5263758
Nuclear magnetic resonance spectroscopy. Benzene-13C.
J Am Chem Soc. 1967 Jun 7;89(12):2967-9 PMID: 6043812
Proton magnetic resonance studies of ribose dinucleoside monophoshates in aqueous solution. I. The nature of the base-stacking interaction in adenylyl 3'--5')adenosine.
J Am Chem Soc. 1969 Jan 1;91(1):168-83 PMID: 5782457
Natural-abundance C nuclear magnetic resonance spectra of medium-molecular-weight organic compounds.
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1152-5 PMID: 16591674

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.65.1.19

Published

1970-01-00

Pages

19-26

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC286184

Subset

External Links

PubMed source DOI: 10.1073/pnas.65.1.19 PMC Full Text View journal details

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Nuclear magnetic resonance spectroscopy: 13C spectra of some common nucleotides.

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