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PMID: 5263774 Published · ppublish English Journal Article

Decay of fluorescence emission anisotropy of the ethidium bromide-DNA complex. Evidence for an internal motion in DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 65 ·No. 2 ·1970-02-00 ·Pages 417-21

Wahl P, Paoletti J, Le Pecq JB

Abstract

Evidence for an internal oscillatory Brownian motion in the DNA helix is obtained from the measurement of the decay of the fluorescence emission anisotropy of the ethidium bromide-DNA complex. The amplitude of the oscillation is found to be equal to 35 degrees and the relaxation time equal to 28 nanoseconds.

MeSH Terms
Birefringence DNA Energy Transfer Fluorescence Phenanthridines Rotation Time Factors
Chemicals
Phenanthridines DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wahl P
Paoletti J
Le Pecq J B
References (5)
5 references, click to expand
  1. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.
    J Mol Biol. 1967 Jul 14;27(1):87-106 PMID: 6033613
  2. Fluorescence spectroscopy of proteins.
    Science. 1968 Nov 1;162(3853):526-33 PMID: 5706935
  3. Flow dichroism of DNA: a new apparatus and further studies.
    Biopolymers. 1969;7(3):335-52 PMID: 5771564
  4. [Study of the polarized fluorescence decay of rabbit gamma globulin conjugated with fluroescein isothiocyanate].
    Biochim Biophys Acta. 1969 Jul 1;181(2):373-80 PMID: 4183023
  5. Relaxation studies of the proflavine-DNA complex: the kinetics of an intercalation reaction.
    J Mol Biol. 1969 Feb 14;39(3):461-77 PMID: 5390557
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.65.2.417
Published
1970-02-00
Pages
417-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282919
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.65.2.417 PMC Full Text View journal details
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