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PMID: 5264136 Published · ppublish English Journal Article

Pre-fork synthesis: a model for DNA replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 3 ·1969-11-00 ·Pages 1065-71

Haskell EH, Daverin CI

Abstract

A model of DNA replication is presented in which DNA synthesis is continuously initiated from parental strand nicks and occurs, with conservation of helix winding number, ahead of the so-called replicating fork. The fork in this model is the locus of unwinding of already replicated, but presumably unstable, DNA. The model, involving Okazaki's notion of multiple initiation, is based upon the properties of Kornberg's DNA polymerase and accounts for the presence of single-stranded nascent DNA fragments in cell lysates. In addition to acting as sites of initiation, the parental strand nicks are implicated as sites of free rotation allowing unwinding of the replicated DNA.

MeSH Terms
DNA/biosynthesis DNA Replication Deoxyribonucleases Ligases Models, Chemical Polynucleotides
Chemicals
Polynucleotides DNA Deoxyribonucleases Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Haskell E H
Daverin C I
References (38)
38 references, click to expand
  1. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.
    J Mol Biol. 1965 Feb;11:373-90 PMID: 14290352
  2. Replication of double-strand nucleic acids.
    Proc Natl Acad Sci U S A. 1965 Jun;53(6):1451-5 PMID: 5217647
  3. Deoxyribonucleic acid secondary structure in the region of the replication point.
    J Mol Biol. 1966 May;17(1):1-9 PMID: 4960115
  4. The enzymatic cleavage of phosphate termini from polynucleotides.
    J Biol Chem. 1967 Mar 10;242(5):936-50 PMID: 4289819
  5. A unique property of the replicating region of chromosomal DNA.
    Biochim Biophys Acta. 1966 Dec 21;129(3):651-4 PMID: 5337977
  6. Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.
    Proc Natl Acad Sci U S A. 1967 Apr;57(4):1021-8 PMID: 5340583
  7. Enzymatic joining of DNA strands: a novel reaction of diphosphopyridine nucleotide.
    Proc Natl Acad Sci U S A. 1967 Jun;57(6):1841-8 PMID: 4291949
  8. The enzymatic repair of DNA. I. Formation of circular lambda-DNA.
    Proc Natl Acad Sci U S A. 1967 Jul;58(1):240-7 PMID: 5341057
  9. Linkage of polynucleotides through phosphodiester bonds by an enzyme from Escherichia coli.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1426-33 PMID: 5341238
  10. Polynucleotide cellulose as a substrate for a polynucleotide ligase induced by phage T4.
    Biochem Biophys Res Commun. 1967 Aug 23;28(4):578-86 PMID: 6052493
  11. Effect of the methionine analogs, ethionine and norleucine, on DNA synthesis in Escherichia coli 15T.
    J Mol Biol. 1968 Feb 14;31(3):401-14 PMID: 4866332
  12. The enzymatic repair of DNA, II. Characterization of phage-induced sealase.
    Proc Natl Acad Sci U S A. 1967 Nov;58(5):1996-2003 PMID: 4295584
  13. Enzymatic reactions at termini of DNA.
    J Cell Physiol. 1967 Oct;70(2):Suppl:181-99 PMID: 4295791
  14. On the mechanism of DNA replication in mammalian chromosomes.
    J Mol Biol. 1968 Mar 14;32(2):327-41 PMID: 5689363
  15. Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.
    Proc Natl Acad Sci U S A. 1968 Feb;59(2):598-605 PMID: 4967086
  16. Studies of DNA replication in vivo. I. Isolation of the first intermediate of DNA replication in bacteria as single-stranded DNA.
    Proc Natl Acad Sci U S A. 1968 May;60(1):329-36 PMID: 4968634
  17. DNA repair.
    Annu Rev Biochem. 1968;37:175-200 PMID: 4875714
  18. Exonuclease VI, a new nuclease activity associated with E. coli DNA polymerase.
    Proc Natl Acad Sci U S A. 1968 Jul;60(3):943-50 PMID: 4875807
  19. Role of polynucleotide ligase in T4 DNA replication.
    J Mol Biol. 1968 Aug 14;35(3):639-42 PMID: 4877007
  20. Mechanism of DNA chain growth, II. Accumulation of newly synthesized short chains in E. coli infected with ligase-defective T4 phages.
    Proc Natl Acad Sci U S A. 1968 Aug;60(4):1356-62 PMID: 4299945
  21. Active center of DNA polymerase.
    Science. 1969 Mar 28;163(3874):1410-8 PMID: 4304249
  22. Intermediates in T4 DNA replication in a T4 ligase deficient strain.
    Cold Spring Harb Symp Quant Biol. 1968;33:145-50 PMID: 4891959
  23. Studies on the joining of DNA by polynucleotide ligase of phage T4.
    Cold Spring Harb Symp Quant Biol. 1968;33:151-64 PMID: 4891960
  24. Analysis of DNA fine secondary structure in relation to replication and transcription.
    Cold Spring Harb Symp Quant Biol. 1968;33:179-86 PMID: 4977753
  25. Endonuclease II of E. coli.
    Cold Spring Harb Symp Quant Biol. 1968;33:271-5 PMID: 4891969
  26. DNA replication: the rolling circle model.
    Cold Spring Harb Symp Quant Biol. 1968;33:473-84 PMID: 4891987
  27. DNA replication after T4 infection.
    Cold Spring Harb Symp Quant Biol. 1968;33:485-93 PMID: 5254576
  28. DNA polymerase and the cell membrane after T4 infection.
    Cold Spring Harb Symp Quant Biol. 1968;33:495-500 PMID: 4891988
  29. Initiation and propagation of growing points in the DNA of phage T4.
    Cold Spring Harb Symp Quant Biol. 1968;33:501-7 PMID: 4891989
  30. Synthesis and maturation of phage P22 DNA. I. Identification of intermediates.
    J Mol Biol. 1968 Jun 28;34(3):621-41 PMID: 4938561
  31. Biologic synthesis of deoxyribonucleic acid.
    Science. 1960 May 20;131(3412):1503-8 PMID: 14411056
  32. The molecular synchrony and sequential replication of DNA in Escherichia coli.
    Proc Natl Acad Sci U S A. 1963 Apr;49:551-9 PMID: 13937133
  33. Changes in the physical state of DNA during the replication cycle.
    Proc Natl Acad Sci U S A. 1963 Mar 15;49:386-92 PMID: 13982522
  34. Sequential replication of the Bacillus subtilis chromosome. II. Isotopic transfer experiments.
    Proc Natl Acad Sci U S A. 1963 Jun;49:806-13 PMID: 14002701
  35. The bacterial chromosome and its manner of replication as seen by autoradiography.
    J Mol Biol. 1963 Mar;6:208-13 PMID: 14017761
  36. A THEORY OF CROSSING-OVER BY MEANS OF HYBRID DEOXYRIBONUCLEIC ACID.
    Nature. 1963 Sep 14;199:1034-40 PMID: 14066935
  37. ISOLATION OF THE GROWING POINT IN THE BACTERIAL CHROMOSOME.
    Proc Natl Acad Sci U S A. 1964 Jul;52:125-32 PMID: 14192649
  38. A COMPARISON OF THE INITIAL ACTIONS OF SPLEEN DEOXYRIBONUCLEASE AND PANCREATIC DEOXYRIBONUCLEASE.
    J Biol Chem. 1965 Mar;240:1274-80 PMID: 14284736
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.64.3.1065
Published
1969-11-00
Pages
1065-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC223344
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.64.3.1065 PMC Full Text View journal details
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