Synthesis of proteins in cells infected with herpesvirus, VI. Characterization of the proteins of the viral membrane.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 66 ·No. 3 ·1970-07-00 ·Pages 799-806

Kaplan AS, Ben-Porat T

Abstract

The envelope of pseudorabies virions can be removed by treatment with Triton X-100, leaving the nucleocapsid intact. The viral envelope removed in this fashion contains all the glycoproteins and about 40 per cent of the proteins of the virions. Analysis by polyacrylamide gel electrophoresis revealed that the envelope proteins migrate as four distinct peaks, all of which contain glycoproteins. The proteins of the nucleocapsids migrate in the gels as six distinct peaks.

MeSH Terms

Animals Carbon Isotopes Culture Techniques DNA, Viral/analysis Electrophoresis, Disc Glucosamine/metabolism Glycoproteins/analysis Herpesviridae/analysis Hydrogen-Ion Concentration Kidney Leucine/metabolism Membranes/metabolism Microscopy, Electron Rabbits Surface-Active Agents Thymidine/metabolism Tritium Viral Proteins/analysis,biosynthesis

Chemicals

Carbon Isotopes DNA, Viral Glycoproteins Surface-Active Agents Viral Proteins Tritium Leucine Glucosamine Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kaplan A S

Ben-Porat T

References (14)

14 references, click to expand

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J Virol. 1970 Feb;5(2):123-31 PMID: 4315403
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J Virol. 1968 May;2(5):517-36 PMID: 4301317
Amino acid metabolism in mammalian cell cultures.
Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
Synthesis of proteins in cells infected with herpesvirus. V. Viral glycoproteins.
Virology. 1970 Jun;41(2):265-73 PMID: 4987226
Electron microscopy of herpes simplex virus. IV. Studies with ferritin-conjugated antibodies.
J Virol. 1968 Oct;2(10):1172-84 PMID: 4302020
Synthesis of proteins in cells infected with herpesvirus. 3. Relative amino acid content of various proteins formed after infection.
Virology. 1970 Jan;40(1):90-101 PMID: 5411196
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Virology. 1957 Dec;4(3):435-57 PMID: 13507306
Cellular membranes: the biosynthesis of glycoprotein and glycolipid in hela cell membranes.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.66.3.799

Published

1970-07-00

Pages

799-806

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283121

Subset

External Links

PubMed source DOI: 10.1073/pnas.66.3.799 PMC Full Text View journal details

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Synthesis of proteins in cells infected with herpesvirus, VI. Characterization of the proteins of the viral membrane.

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