Suppression of chemical mutagenesis in bacteriophage T4 by genetically modified DNA polymerases.
Two antimutagenic DNA polymerases of bacteriophage T4 markedly reduce transition mutagenesis by a variety of chemical mutagens. Spontaneous mutation and mutagenesis by 2-aminopurine, 5-bromodeoxyuridine, and thymine deprivation are strongly suppressed. Mutagenesis at G:C sites by ethyl methanesulfonate, and at A:T sites by nitrous acid, is moderately suppressed. Mutagenesis at G:C sites by hydroxylamine and by nitrous acid is not suppressed. These results support the notion that the indispensable DNA polymerase of bacteriophage T4 plays a crucial role in the selection of the correct base during DNA replication. The data also reveal that mutagenic specificities of chemical agents depend as much upon the characteristics of the enzymatic apparatus of DNA replication as they do upon the chemistry of primary mutational lesions.
17 references, click to expand
-
Active center of DNA polymerase.Science. 1969 Mar 28;163(3874):1410-8 PMID: 4304249
-
On the specificity of DNA polymerase.Proc Natl Acad Sci U S A. 1967 Mar;57(3):650-7 PMID: 16591513
-
Characteristics of mutations appearing spontaneously in extracellular particles of bacteriophage T4.Genetics. 1967 Mar;55(3):387-98 PMID: 5341474
-
Spontaneous mutations accumulating in bacteriophage T4 in the complete absence of DNA replication.Proc Natl Acad Sci U S A. 1966 Apr;55(4):738-43 PMID: 5219679
-
Deoxyribonucleic acid synthesis in Escherichia coli infected with some deoxyribonucleic acid polymerase-less mutants of bacteriophage T4.Virology. 1966 Jan;28(1):100-7 PMID: 4285470
-
Repair and recombination in phage T4. II. Genes affecting UV sensitivity.Cold Spring Harb Symp Quant Biol. 1968;33:333-8 PMID: 4891973
-
The function of T4 DNA polymerase.Cold Spring Harb Symp Quant Biol. 1968;33:345-50 PMID: 4891974
-
Antimutagenic DNA polymerases of bacteriophage T4.Cold Spring Harb Symp Quant Biol. 1968;33:339-44 PMID: 5254574
-
Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4.J Biol Chem. 1968 Feb 10;243(3):627-38 PMID: 4866523
-
Comparative rates of spontaneous mutation.Nature. 1969 Mar 22;221(5186):1132 PMID: 4378427
-
On the role of DNA polymerase in base selection.Cold Spring Harb Symp Quant Biol. 1966;31:693-7 PMID: 5237208
-
Tautomeric equilibria in inosine and adenosine.J Mol Biol. 1969 Mar 14;40(2):307-10 PMID: 5365014
-
Genetic control of mutation rates in bacteriophageT4.Nature. 1969 Mar 22;221(5186):1128-32 PMID: 4975273
-
Isolation of an E. coli strain with a mutation affecting DNA polymerase.Nature. 1969 Dec 20;224(5225):1164-6 PMID: 4902142
-
Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase.Nature. 1969 Dec 20;224(5225):1166-8 PMID: 4902143
-
Mutagenic DNA polymerase.Biochem Biophys Res Commun. 1965 Oct 8;21(1):6-8 PMID: 5865492
-
The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5.Proc Natl Acad Sci U S A. 1965 Oct;54(4):1241-8 PMID: 5219829
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
