Demonstration of delayed hypersensitivity to soluble antigens of chemically induced tumors by inhibition of macrophage migration.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 4 ·1969-12-00 ·Pages 1176-80

Bloom BR, Bennett B, Oettgen HF, McLean EP, Old LJ

Abstract

Sensitization to soluble antigens of chemically induced tumors is demonstrable in syngeneic strain 13 (inbred) guinea pigs by specific inhibition of macrophage migration. According to their capacity to (a) inhibit the migration of peritoneal cells from sensitized strain 13 donors or (b) release migration-inhibitory factor from their lymph node cells, the soluble antigens of three different chemically induced tumors did not cross react. This is in keeping with the absence of cross-reactivity among the same three tumors in previous tests of transplantation resistance or delayed hypersensitivity in vivo. Thus, inhibition of macrophage migration may provide a feasible assay in vitro for use in defining these soluble antigens further by physicochemical methods.

MeSH Terms

Animals Antigens Benz(a)Anthracenes Guinea Pigs Hypersensitivity, Delayed Inbreeding Macrophages/physiology Methylcholanthrene Sarcoma, Experimental/chemically induced,immunology

Chemicals

Antigens Benz(a)Anthracenes Methylcholanthrene

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Bloom B R

Bennett B

Oettgen H F

McLean E P

Old L J

References (11)

11 references, click to expand

Mechanism of a reaction in vitro associated with delayed-type hypersensitivity.
Science. 1966 Jul 1;153(3731):80-2 PMID: 5938421
Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction.
Proc Natl Acad Sci U S A. 1966 Jul;56(1):72-7 PMID: 5229858
Reactions in vivo and in vitro produced by a soluble substance associated with delayed-type hypersensitivity.
Proc Natl Acad Sci U S A. 1968 Mar;59(3):756-62 PMID: 5238658
Delayed hypersensitivity and transplantation immunity elicited by soluble antigens of chemically induced tumours in inbred guinea-pigs.
Nature. 1968 Oct 19;220(5164):295-7 PMID: 5684864
Tumor-specific antigens detected by inhibition of macrophage migration.
Science. 1969 Jul 18;165(3890):296-7 PMID: 4306915
In vitro detection of guinea pig alloantigens by the macrophage-inhibition technique.
Transplantation. 1969 Oct;8(4):485-9 PMID: 5267515
Migration inhibitory factor associated with delayed-type hypersensitivity.
Fed Proc. 1968 Jan-Feb;27(1):13-5 PMID: 4952930
Demonstration of delayed (tuberculin) type hypersensitivity in vitro. II. Specific reaction of hypersensitive cells with antigen.
Z Immun exp ther. 1961 Dec;122:420-36 PMID: 13918676
In vitro cell migration as a model for delayed hypersensitivity.
Proc Soc Exp Biol Med. 1962 Nov;111:514-21 PMID: 13947220
DELAYED HYPERSENSITIVITY IN VITRO. I. THE SPECIFICITY OF INHIBITION OF CELL MIGRATION BY ANTIGENS.
J Immunol. 1964 Aug;93:264-73 PMID: 14205621
IN VITRO STUDIES ON HOMOGRAFT SENSITIVITY.
Nature. 1965 Feb 27;205:916-7 PMID: 14293156

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.64.4.1176

Published

1969-12-00

Pages

1176-80

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC223265

Subset

External Links

PubMed source DOI: 10.1073/pnas.64.4.1176 PMC Full Text View journal details

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Demonstration of delayed hypersensitivity to soluble antigens of chemically induced tumors by inhibition of macrophage migration.

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