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PMID: 5276766 Published · ppublish English Journal Article

The leader sequence from the 5'-terminus to the A-protein initiation codon in MS2-virus RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 3 ·1971-03-00 ·Pages 585-9

De Wachter R, Vandenberghe A, Merregaert J, Contreras R, Fiers W

Abstract

RNA fragments of different chain length, each containing the 5'-terminal guanosine tetraphosphate (pppGp) of bacteriophage-MS2 RNA, have been isolated from partial ribonuclease digests of the viral RNA. The longest fragment overlaps with the ribosomalbinding site of the A-protein cistron. The base sequence has been established for the major part. The results directly confirm that the A-protein cistron is closest to the 5'-terminus. Its initiating (AUG) codon starts at position 130, being preceded by an untranslated sequence of 129 nucleotides.

MeSH Terms
Autoradiography Coliphages
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
De Wachter R
Vandenberghe A
Merregaert J
Contreras R
Fiers W
References (12)
12 references, click to expand
  1. Nucleotide sequence from the polypeptide chain termination region of the coat protein cistron in bacteriophage R17 RNA.
    Nature. 1970 Jan 10;225(5228):147-51 PMID: 5409960
  2. The specific cleavage of yeast ribosomal ribonucleic acid with nucleases.
    Biochemistry. 1966 Mar;5(3):988-93 PMID: 5330071
  3. Studies on the bacteriophage MS2. I. Distribution of purine sequences in the viral RNA and in yeast RNA.
    J Mol Biol. 1965 Sep;13(2):432-50 PMID: 5867029
  4. Nucleotide sequence from the coat protein cistron of R17 bacteriophage RNA.
    Nature. 1969 Sep 6;223(5210):1009-14 PMID: 5811898
  5. Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA.
    Nature. 1969 Dec 6;224(5223):957-64 PMID: 5360547
  6. Studies on the bacteriophage MS2. V. The 5'-terminal tetranucleotide sequence of the viral RNA chain.
    FEBS Lett. 1968 Aug;1(2):93-96 PMID: 11945263
  7. Gene order in the bacteriophage R17 RNA: 5'-a protein-coat protein-synthetase-3'.
    Nature. 1970 Apr 18;226(5242):230-7 PMID: 5437513
  8. Gene order of RNA bacteriophage M 12.
    Nature. 1970 May 16;226(5246):604-7 PMID: 4912788
  9. Untranslated nucleotide sequence at the 5'-end of R17 bacteriophage RNA.
    Nature. 1970 Aug 8;227(5258):570-4 PMID: 5429288
  10. Limited enzymatic degradation of bacteriophage MS2 RNA.
    Arch Int Physiol Biochim. 1968 Feb;76(1):194-5 PMID: 4175043
  11. Sequence of the first 175 nucleotides from the 5' terminus of Qbeta RNA synthesized in vitro.
    Nature. 1969 Dec 13;224(5224):1083-6 PMID: 5353716
  12. The specific cleavage of ribonucleic acid from reticulocyte ribosomal subunits.
    Biochemistry. 1966 Mar;5(3):1103-8 PMID: 5911289
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.3.585
Published
1971-03-00
Pages
585-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388995
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.3.585 PMC Full Text View journal details
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