The mechanism of protein synthesis activation after fertilization of sea urchin eggs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 3 ·1971-03-00 ·Pages 600-4

Metafora S, Felicetti L, Gambino R

Abstract

Upon fertilization of Paracentrotus lividus eggs, the ability of their ribosomes to form a ternary complex with poly(U) and phenylalanyl-tRNA is enhanced. In addition, it is possible to extract from ribosomes of the unfertilized egg a protein factor that inhibits polyphenylalanine synthesis by decreasing the binding of mRNA and aminoacyl-tRNA to the ribosomes. The observed increase in the rate of protein synthesis that occurs at fertilization could be accounted for by the removal of this inhibitory protein from the ribosomes.

MeSH Terms

Animals Carbon Isotopes Cell-Free System

Chemicals

Carbon Isotopes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Metafora S

Felicetti L

Gambino R

References (11)

11 references, click to expand

Polynucleotide attachment to ribosomes.
J Mol Biol. 1966 Jun;18(1):8-20 PMID: 5337560
Stimulation of phenylalanine incorporation by polyuridylic acid in homogenates of sea urchin eggs.
Biochem Biophys Res Commun. 1962 Oct 31;9:313-7 PMID: 14001107
Experimentally induced activation of the ribosomes of the unfertilized sea urchin egg.
Proc Natl Acad Sci U S A. 1965 Jul;54(1):107-11 PMID: 5216344
Interrelation of messenger polyribonucleotides and ribosomes in the sea urchin egg during embryonic development.
Biochem Biophys Res Commun. 1962 Aug 31;8:511-5 PMID: 14478972
The protein-synthetic lesion in unfertilized eggs.
Proc Natl Acad Sci U S A. 1967 Mar;57(3):735-42 PMID: 16591525
The use of bentonite in the isolation of plant polyribosomes.
Biochim Biophys Acta. 1967;145(3):828-31 PMID: 6065698
Activation of ribosomes in sea urchin eggs in response to fertilization.
Exp Cell Res. 1961 Nov;25:405-17 PMID: 14449935
ON THE ROLE OF AMMONIUM OR POTASSIUM ION IN AMINO ACID POLYMERIZATION.
Proc Natl Acad Sci U S A. 1964 Jun;51:1216-20 PMID: 14215647
Comparison of amino acid polymerization factors isolated from rat liver and rabbit reticulocytes.
Arch Biochem Biophys. 1968 May;125(2):548-57 PMID: 4297868
Polyribosome formation and RNA synthesis in the early post-fertilization stages of the sea urchin egg.
Dev Biol. 1969 Jan;19(1):73-86 PMID: 5812947
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.3.600

Published

1971-03-00

Pages

600-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388998

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.3.600 PMC Full Text View journal details

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