Home LiteratureArticle Details
PMID: 5283944 Published · ppublish English Journal Article

Transplantation of nucle in Drosophila melanogaster.

Zalokar M

Abstract

Nuclei surrounded by ooplasm of the syncytial stage of developing eggs of wild-type Drosophila melanogaster were implanted into freshly laid fertilized eggs of females of a y w stock. More than half of the recipient eggs produced larvae, but few of the larvae hatched or developed further. The best sets of experiments gave about twelve percent of imagos, mostly y w in appearance. Several larvae were mosaics with yellow Malpighian tubes, and two flies had part of the abdominal segments of the wild type. Half of the flies were fertile, but they produced only y w offspring, except for two males that had y w appearance, but wild-type gonads. When crossed with y w females, they gave wild-type females and y w males.

MeSH Terms
Animals Drosophila/embryology,growth & development Female Fertility Genotype Larva/growth & development Male Micromanipulation Mosaicism Nuclear Transfer Techniques Phenotype Transplantation, Homologous
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Zalokar M
References (5)
5 references, click to expand
  1. Transplantation of embryonic nuclei into unfertilized eggs of Drosophila melanogaster.
    Nature. 1968 Sep 21;219(5160):1268-9 PMID: 5677428
  2. Transplantation of Living Nuclei From Blastula Cells into Enucleated Frogs' Eggs.
    Proc Natl Acad Sci U S A. 1952 May;38(5):455-63 PMID: 16589125
  3. Imaginal structures after nuclear transplantation in Drosophila melanogaster.
    Naturwissenschaften. 1970 Nov;57(11):550-1 PMID: 5531653
  4. Experiments on nuclear transplantation in Drosophila melanogaster. Preliminary report.
    Rev Suisse Zool. 1967 Dec;74(3):614-5 PMID: 6081342
  5. Nuclear transplantation in Drosophila melanogaster.
    Nature. 1971 Mar 19;230(5290):185-6 PMID: 4925795
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1971-07-00
Pages
1539-41
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389235
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]