Home LiteratureArticle Details

PMID: 52873 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Absence of H-2 antigens capable of reacting with cytotoxic T cells on a teratoma line expressing a T/t locus antigen.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 9 ·1975-09-00 ·Pages 3661-5

Forman J, Vitetta ES

Abstract

An established cell line of murine teratoma cells (F9), which lacks serologically detectable H-2 that is determined by a wild-type T/t locus gene. These cells are not killed and do not react with cytotoxic T cells sensitized to H-2 antigens in a cell-mediated lympholysis assay. Modification of spleen cells from the strain or origin (129) of this teratoma line with trinitrobenzene sulfonic acid allows the generation of syngeneic killer cells that display a cytotoxic effect against trinitrophenyl-modified splenic targets, but not against trinitrophenyl-modified F9 targets. Thus, the F9 antigen is structurally similar to H-2b but does not act as a target antigen in the cell-mediated lympholysis assay for anti-H-2b cytotoxic T cells, nor does it crossreact with H-2b antigens at the T cell level.

MeSH Terms

Animals Cell Line Cytotoxicity Tests, Immunologic Epitopes Histocompatibility Antigens/analysis Mice Nitrobenzenes/immunology Spleen/immunology T-Lymphocytes/immunology Teratoma/immunology

Chemicals

Epitopes Histocompatibility Antigens Nitrobenzenes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Forman J

Vitetta E S

References (23)

23 references, click to expand

Structural similarities between a product of the T/t-locus isolated from sperm and teratoma cells, and H-2 antigens isolated from splenocytes.
Proc Natl Acad Sci U S A. 1975 Aug;72(8):3215-9 PMID: 1059107
On the role of the H-2 histocompatibility complex in determining the specificity of cytotoxic effector cells sensitized against syngeneic trinitrophenyl-modified targets.
J Exp Med. 1975 Aug 1;142(2):403-18 PMID: 1079848
Cell interactions between histoincompatible T and B lymphocytes. VII. Cooperative responses between lymphocytes are controlled by genes in the I region of the H-2 complex.
J Exp Med. 1975 Jan 1;141(1):263-8 PMID: 1090698
Studies on transplantable testicular teratomas of strain 129 mice.
J Natl Cancer Inst. 1958 Jun;20(6):1257-75 PMID: 13549983
ABNORMALITIES ASSOCIATED WITH A CHROMOSOME REGION IN THE MOUSE. II. EMBRYOLOGICAL EFFECTS OF LETHAL ALLELES IN THE T-REGION.
Science. 1964 Apr 17;144(3616):263-7 PMID: 14169710
Quantitative studies of the sensitivity of normal and neoplastic mouse cells to the cytotoxic action of isoantibodies.
J Exp Med. 1962 Mar 1;115:527-53 PMID: 14475217
Effect of antigen density on complement-mediated lysis, T-cell-mediated killing, and antigenic modulation.
J Natl Cancer Inst. 1974 Dec;53(6):1759-65 PMID: 4140238
Allograft response in vitro.
Transplant Rev. 1972;12:91-140 PMID: 4143965
Cell-mediated lympholysis. Importance of serologically defined H-2 regions.
J Exp Med. 1973 May 1;137(5):1303-9 PMID: 4267208
Generation of cytotoxic lymphocytes in mixed lymphocyte reactions. I. Specificity of the effector cells.
J Exp Med. 1973 Sep 1;138(3):672-85 PMID: 4269560
Cell-mediated cell lysis in vitro: genetic control of killer cell production and target specificities in the mouse.
Eur J Immunol. 1974 May;4(5):378-87 PMID: 4277395
Surface antigens common to mouse cleavage embryos and primitive teratocarcinoma cells in culture.
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2988-92 PMID: 4355379
Cell surface antigens of a mouse testicular teratoma. Identification of an antigen physically associated with H-2 antigens on tumor cells.
J Exp Med. 1974 Jul 1;140(1):61-78 PMID: 4365513
Function of macrophages in antigen recognition by guinea pig T lymphocytes. I. Requirement for histocompatible macrophages and lymphocytes.
J Exp Med. 1973 Nov 1;138(5):1194-212 PMID: 4542806
Cell-mediated cytotoxicity to trinitrophenyl-modified syngeneic lymphocytes.
Eur J Immunol. 1974 Aug;4(8):527-33 PMID: 4547153
Studies on the induction and expression of T cell-mediated immunity. II. Antiserum blocking of cell-mediated cytolysis.
J Immunol. 1974 Apr;112(4):1308-21 PMID: 4592598
Cell-mediated lympholysis of trinitrophenyl-modified autologous lymphocytes. Effector cell specificity to modified cell surface components controlled by H-2K and H-2D serological regions of the murine major histocompatibility complex.
J Exp Med. 1975 Jun 1;141(6):1348-64 PMID: 47900
Letter: Absence of serologically detectable H-2 on primitive teratocarcinoma cells in culture.
Transplantation. 1974 Jun;17(6):632-4 PMID: 4829671
Histocompatibility genes of mice. XI. Evidence establishing a new histocompatibility locus, H-12, and new H-2 allele, H-2bc.
Transplantation. 1971 Jun;11(6):525-30 PMID: 4931454
In vitro growth and differetiation of clonal populations of multipotential mouse clls derived from a transplantable testicular teratocarcinoma.
J Natl Cancer Inst. 1970 May;44(5):1001-14 PMID: 4936569
Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.
Immunology. 1968 Feb;14(2):181-96 PMID: 4966657
Cell mediated immunity induced in vitro.
Nature. 1970 Oct 3;228(5266):62-3 PMID: 5456212
Developmental potentialities of clonal in vitro cultures of mouse testicular teratoma.
J Natl Cancer Inst. 1970 May;44(5):1015-36 PMID: 5514468

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.9.3661

Published

1975-09-00

Pages

3661-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433056

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.9.3661 PMC Full Text View journal details

Analysis Services

Absence of H-2 antigens capable of reacting with cytotoxic T cells on a teratoma line expressing a T/t locus antigen.

Contact

WeChat Official Account