Migration of mouse 3T3 fibroblasts in response to a serum factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 11 ·1971-11-00 ·Pages 2799-801

Lipton A, Klinger I, Paul D, Holley RW

Abstract

A serum factor that promotes migration of (mouse) 3T3 fibroblast cells is shown to be distinct from the growth-promoting and cell-survival factors of serum. The factor promotes migration of cells from the edge of a wound in confluent 3T3 cells, but cell migration under these conditions does not lead to the initiation of DNA synthesis. Subsequent addition of serum initiates DNA synthesis in the migrating cells. The results establish that breaking contacts between quiescent 3T3 cells is not sufficient to initiate DNA synthesis. The DNA synthesis observed in migrating 3T3 cells in the typical "woundhealing" experiment presumably results because the migrating cells have an increased ability to use serum factors. Serum-factor requirements for "wound healing" in cultured 3T3 cells are discussed.

MeSH Terms

Animals Blood Proteins/pharmacology Cell Line Cell Movement Cell Survival Cells, Cultured Chromatography, Gel Culture Media DNA/biosynthesis Fibroblasts/drug effects,metabolism Growth/drug effects Mice Stimulation, Chemical Thymidine/metabolism Time Factors Tritium Wound Healing/drug effects

Chemicals

Blood Proteins Culture Media Tritium DNA Thymidine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lipton A

Klinger I

Paul D

Holley R W

References (10)

10 references, click to expand

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Nature. 1970 Aug 22;227(5260):798-801 PMID: 4317353
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Nature. 1970 Aug 22;227(5260):802-6 PMID: 4317354
Proliferative activity and cytochemical properties of nuclear chromatin related to local cell density of epithelial cells.
Exp Cell Res. 1970 Sep;62(1):262-70 PMID: 5481249
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Proc Natl Acad Sci U S A. 1970 May;66(1):204-10 PMID: 5273897
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Exp Cell Res. 1971 Jan;64(1):156-62 PMID: 5541962
Serum factor requirements of normal and simian virus 40-transformed 3T3 mouse fibroplasts.
Proc Natl Acad Sci U S A. 1971 Mar;68(3):645-52 PMID: 5276775

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.11.2799

Published

1971-11-00

Pages

2799-801

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389528

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.11.2799 PMC Full Text View journal details

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Migration of mouse 3T3 fibroblasts in response to a serum factor.

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