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PMID: 5289016 Published · ppublish English Journal Article

Ion transport underlying metabolically controlled volume changes of isolated mitochondria.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 2 ·1970-10-00 ·Pages 702-9

Izzard S, Tedeschi H

Abstract

An earlier study has indicated that the swelling of isolated mitochondria induced by inorganic phosphate (P(i)) can be accounted for largely or completely by the accumulation of ions. In the present study, a similar uptake was shown to be induced by a wider range of P(i) concentrations. Addition of ADP, KCN, or 2,4-dinitrophenol initiates a shrinkage which can be accounted for by the efflux of ions. The results are consistent with the explanations that (a) P(i) induces a transport of ions and a concomitant osmotic swelling, and (b) the addition of substances which compete or interfere with the energy available for transport results in ion efflux and a corresponding mitochondrial shrinkage. The results are not consistent with proposals that the changes in the light scattered by mitochondrial suspensions with alterations in metabolic states reflect a mechanochemical coupling phenomenon.

MeSH Terms
Adenine Nucleotides/pharmacology Animals Biological Transport, Active/drug effects Cyanides/pharmacology Dinitrophenols/pharmacology Electron Transport In Vitro Techniques Liver/cytology Magnesium/pharmacology Male Mitochondria/drug effects,metabolism Mitochondrial Swelling Osmolar Concentration Oxidative Phosphorylation Phosphates/pharmacology Polarography Potassium/metabolism,pharmacology Rats Sodium/metabolism Spectrophotometry Water-Electrolyte Balance/drug effects
Chemicals
Adenine Nucleotides Cyanides Dinitrophenols Phosphates Sodium Magnesium Potassium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Izzard S
Tedeschi H
References (30)
30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.67.2.702
Published
1970-10-00
Pages
702-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC283262
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.67.2.702 PMC Full Text View journal details
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