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PMID: 5289238 Published · ppublish English Journal Article

Role of 5S RNA in the functions of 50S ribosomal subunits.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 2932-6

Erdmann VA, Fahnestock S, Higo K, Nomura M

Abstract

50S ribosomal subunits from Bacillus stearothermophilus can be reconstituted from their dissociated components, namely a 5S RNA-free protein fraction, a 5S RNA-free 23S ribosomal RNA fraction, and purified 5S RNA. The biological activity of reconstituted particles in polypeptide synthesis is dependent on the presence of 5S RNA. In the absence of 5S RNA, particles are produced that have greatly reduced activity in (a) polypeptide synthesis directed by synthetic, as well as natural, messenger RNA, (b) peptidyl transferase assay, (c) [(3)H]UAA binding dependent on peptide chain termination factor R1, (d) G factor-dependent [(3)H]GTP binding, and (e) codon-directed tRNA binding assayed in the presence of 30S subunits. Thus, 5S RNA is an essential 50S ribosomal component.

MeSH Terms
Bacillus/enzymology,metabolism Bacterial Proteins/biosynthesis Binding Sites Centrifugation, Density Gradient Guanosine Triphosphate/metabolism Peptide Biosynthesis Peptide Chain Initiation, Translational Peptide Chain Termination, Translational Polynucleotides/metabolism Protein Binding RNA, Bacterial/metabolism,physiology RNA, Messenger/metabolism RNA, Ribosomal/metabolism RNA, Transfer/metabolism Ribosomes/physiology Transferases/metabolism Tritium
Chemicals
Bacterial Proteins Polynucleotides RNA, Bacterial RNA, Messenger RNA, Ribosomal Tritium Guanosine Triphosphate RNA, Transfer Transferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Erdmann V A
Fahnestock S
Higo K
Nomura M
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.12.2932
Published
1971-12-00
Pages
2932-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389563
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.12.2932 PMC Full Text View journal details
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