Kinetics of sodium dodecyl sulfate solubilization of Mycoplasma laidlawii plasma membranes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 9 ·1971-09-00 ·Pages 1996-8

Auborn JJ, Eyring EM, Choules GL

Abstract

The kinetics of sodium dodecyl sulfate solubilization of aqueous suspensions of Mycoplasma laidlawii membranes have been investigated by light scattering in a stopped-flow apparatus. There was evidence of direct interaction between the membranes and sodium dodecyl sulfate micelles above the critical micelle concentration, although of lower order kinetically than with monomeric dodecyl sulfate anions below the critical micelle concentration. The activation energy remained the same in either case, about 10 kcal/mol. Static light-scattering studies at higher resolution showed that the solubilized membranes are in the form of small aggregates.

MeSH Terms

Acholeplasma laidlawii/cytology,drug effects Bacterial Proteins/analysis Cell Membrane/analysis,drug effects Chromatography, Gel Detergents/pharmacology Electrophoresis, Disc Hot Temperature Kinetics Molecular Weight Mycoplasma/drug effects Scattering, Radiation Solubility Spectrophotometry Sulfuric Acids/pharmacology

Chemicals

Bacterial Proteins Detergents Sulfuric Acids

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Auborn J J

Eyring E M

Choules G L

References (13)

13 references, click to expand

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Reassembly of Mycoplasma membranes disaggregated by detergents.
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Physical characteristics of proteins of the electron transfer system and interpretation of the structure of the mitochondrion.
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Proc Natl Acad Sci U S A. 1970 Sep;67(1):120-7 PMID: 5272308
Isolation and characterization of the major protein component of sarcotubular membranes.
Biochemistry. 1970 Jul 7;9(14):2909-17 PMID: 5459544
The gross conformation of protein-sodium dodecyl sulfate complexes.
J Biol Chem. 1970 Oct 10;245(19):5161-5 PMID: 5528242
Gel chromatography of proteins in denaturing solvents. Comparison between sodium dodecyl sulfate and guanidine hydrochloride as denaturants.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.9.1996

Published

1971-09-00

Pages

1996-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389333

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.9.1996 PMC Full Text View journal details

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Kinetics of sodium dodecyl sulfate solubilization of Mycoplasma laidlawii plasma membranes.

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