Home LiteratureArticle Details
PMID: 5289364 Published · ppublish English Journal Article

Regulation of protein synthesis initiation in HeLa cells deprived of single essential amino acids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 9 ·1971-09-00 ·Pages 2057-61

Vaughan MH, Pawlowski PJ, Forchhammer J

Abstract

In HeLa cells deprived of valine, histidine, or methionine initiation of protein synthesis decreases rapidly and disaggregation of polyribosomes also occurs. The mechanism of inhibition does not seem to involve the supply of RNA in the cell, and thus it differs from the initiation of inhibition at elevated temperatures. Polyribosomes rapidly form again if the missing amino acid is restored, even in the presence of actinomycin D.

MeSH Terms
Amino Acids/metabolism Autoradiography Carbon Isotopes Cell Fractionation Centrifugation, Density Gradient Culture Media Dactinomycin/pharmacology HeLa Cells/metabolism Histidine/metabolism Hot Temperature Humans Kinetics Leucine/metabolism Methionine/metabolism Peptide Chain Elongation, Translational Peptide Chain Initiation, Translational Protein Biosynthesis RNA, Ribosomal/biosynthesis Ribosomes/metabolism Time Factors Tritium Uridine/metabolism Valine/metabolism
Chemicals
Amino Acids Carbon Isotopes Culture Media RNA, Ribosomal Tritium Dactinomycin Histidine Methionine Leucine Valine Uridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vaughan M H
Pawlowski P J
Forchhammer J
References (17)
17 references, click to expand
  1. Tryptophan deficiency in rabbit reticulocytes: polyribosomes during interrupted growth of hemoglobin chains.
    Science. 1967 Jan 6;155(3758):83-4 PMID: 6015566
  2. ENTRANCE OF NEWLY FORMED MESSENGER RNA AND RIBOSOMES INTO HELA CELL CYTOPLASM.
    J Mol Biol. 1965 Feb;11:187-201 PMID: 14290358
  3. The effects of methionine deprivation on ribosome synthesis in HeLa cells.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1527-34 PMID: 5237886
  4. Ribosomal subunits of Landschütz ascites cells during changes in polysome distribution.
    Biochim Biophys Acta. 1968 Nov 20;169(1):129-38 PMID: 5727127
  5. Synthesis of ribosomal proteins in the absence of ribosome maturation in methionine-deficient HeLa cells.
    J Mol Biol. 1968 Dec;38(3):431-5 PMID: 5718559
  6. Membrane-bound ribosomes in HeLa cells. I. Their proportion to total cell ribosomes and their association with messenger RNA.
    J Mol Biol. 1969 Aug 28;44(1):47-70 PMID: 4980477
  7. Regulation of protein synthesis in HeLa cells: translation at elevated temperatures.
    J Mol Biol. 1969 Jan;39(2):315-33 PMID: 5362672
  8. Effects of valine deprivation on ribosome formation in HeLa cells.
    J Mol Biol. 1969 Oct 28;45(2):265-75 PMID: 5367028
  9. Regulation of protein synthesis in mammalian cells. II. Inhibition of protein synthesis at the level of initiation during mitosis.
    J Mol Biol. 1970 Jun 28;50(3):655-70 PMID: 5529301
  10. Biosynthetic precursors of 30S and 50S ribosomal particles in Escherichia coli.
    Biochemistry. 1968 Jan;7(1):456-72 PMID: 4991482
  11. Nutritional effects on the polyribosome distribution and rate of protein synthesis in Ehrlich ascites tumor cells in culture.
    J Biol Chem. 1970 Nov 25;245(22):5947-53 PMID: 5484455
  12. The free amino acid pool of cultured human cells.
    J Biol Chem. 1958 Mar;231(1):533-45 PMID: 13538989
  13. Protein turnover in mammaliar cell cultures.
    J Biol Chem. 1959 Mar;234(3):592-7 PMID: 13641266
  14. Amino acid metabolism in mammalian cell cultures.
    Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
  15. Sedimentation characteristics of rapidly labelled RNA from HeLa cells.
    Biochem Biophys Res Commun. 1962 Jun 4;7:486-90 PMID: 14498283
  16. Demonstration of an unstable RNA and of a precursor to ribosomal RNA in HeLa cells.
    Proc Natl Acad Sci U S A. 1963 Feb 15;49:240-8 PMID: 13991616
  17. RNA metabolism in the HeLa cell nucleus.
    J Mol Biol. 1966 May;17(1):117-30 PMID: 5961144
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.9.2057
Published
1971-09-00
Pages
2057-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389350
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.9.2057 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2