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PMID: 5289379 Published · ppublish English Journal Article

Inhibition of the DNA polymerase of Rauscher leukemia virus by single-stranded polyribonucleotides.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 9 ·1971-09-00 ·Pages 2198-202

Tuominen FW, Kenney FT

Abstract

The DNA polymerase of Rauscher murine leukemia virus is strongly and specifically inhibited by nontemplate, single-stranded polyribonucleotides with either the resident viral RNA, native calf-thymus DNA, or poly[d(A-T)] as templates. These inhibitory homopolymers are apparently bound to the template site of the polymerase, since they interact competitively with the template. The strength of the inhibition depends on the particular homopolymer used: poly(U) > poly(G) >> poly(A) > poly(C). The K(i) for poly(U) was 0.08 mug/ml, which represents an apparent affinity six times greater than that observed for viral RNA. No such inhibition was observed with a highly purified DNA polymerase from mouse embryos or the Escherichia coli enzyme.

MeSH Terms
Adenine Nucleotides/pharmacology Buffers Cytosine Nucleotides/pharmacology DNA Nucleotidyltransferases/antagonists & inhibitors,isolation & purification DNA, Viral/metabolism Guanine Nucleotides/pharmacology Hydrogen-Ion Concentration Kinetics Polynucleotides/pharmacology Rauscher Virus/enzymology Ribonucleases/pharmacology Ribonucleotides/pharmacology Templates, Genetic Uracil Nucleotides/pharmacology
Chemicals
Adenine Nucleotides Buffers Cytosine Nucleotides DNA, Viral Guanine Nucleotides Polynucleotides Ribonucleotides Uracil Nucleotides DNA Nucleotidyltransferases Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tuominen F W
Kenney F T
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18 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.9.2198
Published
1971-09-00
Pages
2198-202
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389384
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.9.2198 PMC Full Text View journal details
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