Use of dialyzing culture technique for high yield of Mycoplasma.

Pollock, Mary E. (University of Minnesota, Minneapolis). Use of dialyzing culture technique for high yield of Mycoplasma. J. Bacteriol. 90:1682-1685. 1965.-The saprophytic pleuropneumonia-like organism, Mycoplasma laidlawii, type A, was cultivated within a dialyzing membrane suspended in a reservoir of uninoculated medium. Generation time was not decreased by this method, but the period of active growth was prolonged, and the final yield was 10-fold that attained by the usual cultural methods. A defined mixture, which alone would not support growth of Mycoplasma, could replace the complex soy peptone-yeast extract medium in the inner membrane vessel of the dialyzing culture flask with no decrease in growth rate or yield.