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PMID: 5326110 Published · ppublish English Journal Article

Methylated bases in the host-modified deoxyribonucleic acid of Escherichia coli and bacteriophage lambda.

Journal of bacteriology ·Vol. 91 ·No. 4 ·1966-04-00 ·Pages 1460-8

Gough M, Lederberg S

Abstract

Gough, Michael (Brown University, Providence, R.I.), and Seymour Lederberg. Methylated bases in the host-modified deoxyribonucleic acid of Escherichia coli and bacteriophage lambda. J. Bacteriol. 91:1460-1468. 1966.-The deoxyribonucleic acid (DNA) from strains of Escherichia coli and phage lambda was examined to determine whether the types or amounts of methionine-derived methylated bases present correlated with the host-specific modification of that DNA. The DNA of strain C600 (which has K-12 modification specificity) and of a modificationless mutant of C600 are similar in their content of 5-methylcytosine and 6-methylaminopurine. Strains Bc251 and its P1-lysogen differ in P1-controlled specificity, but they have the same content of 6-methylaminopurine, and both lack 5-methylcytosine in their DNA. Phage lambda contains the same methylated bases as its host of origin, but in reduced amounts and in different proportions. Although minor amounts of these methylated bases may have importance as a result of their location, the presence of the majority of these methylated bases is irrelevant to the specificity of host modification of DNA.

MeSH Terms
Alkylation Carbon Isotopes Chromatography Coliphages/metabolism Cytosine/metabolism DNA, Bacterial/metabolism DNA, Viral/metabolism Escherichia coli/metabolism In Vitro Techniques Methionine/metabolism Purines/metabolism
Chemicals
Carbon Isotopes DNA, Bacterial DNA, Viral Purines Cytosine Methionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gough M
Lederberg S
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36 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.91.4.1460-1468.1966
Published
1966-04-00
Pages
1460-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC316064
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.91.4.1460-1468.1966 PMC Full Text View journal details
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