Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli.

Journal of bacteriology ·Vol. 93 ·No. 1 ·1967-01-00 ·Pages 237-44

Wallace BJ, Pittard J

Abstract

Mutant strains of Escherichia coli K-12 were isolated possessing mutations which affected the tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase, the phenylalanine-inhibitable DAHP synthetase, or the tryptophan-repressible DAHP synthetase. The mutations causing the loss of each of these activities have been mapped and are widely separated from each other on the E. coli chromosome. Chromatography on diethylaminoethyl cellulose columns allowed the recognition of four peaks of activity.

MeSH Terms

Amino Acids/biosynthesis Chromatography Chromosome Mapping Enzyme Repression Escherichia coli/enzymology,isolation & purification Isoenzymes/analysis Ligases/analysis Mutation Phenylalanine/pharmacology Tryptophan/pharmacology Tyrosine/pharmacology

Chemicals

Amino Acids Isoenzymes Tyrosine Phenylalanine Tryptophan Ligases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wallace B J

Pittard J

References (14)

14 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.93.1.237-244.1967

Published

1967-01-00

Pages

237-44

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC314994

Subset

External Links

PubMed source DOI: 10.1128/jb.93.1.237-244.1967 PMC Full Text View journal details

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Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli.

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