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PMID: 5338562 Published · ppublish English Journal Article

Enzymatic mechanisms of DNA replication.

The Journal of general physiology ·Vol. 49 ·No. 6 ·1966-07-00 ·Pages 59-79

Mitra S, Kornberg A

Abstract

DNA polymerases purified from several sources are characterized by replication of the 3'-hydroxy-terminated strand of a helical template. Failure to achieve simultaneous replication of the 5'-strand leads to aberrations in the synthesized DNA, described as nondenaturability and branching. Aberrations in synthesized DNA were not observed when (a) the 5'-strand was destroyed by a specific nuclease during the course of replication or (b) a single-stranded (circular) phage (M13) DNA served as template. Replication of a single-stranded, circular DNA produced a helical product, but the nature of initiation of a new strand by the circular template remains to be explained. Hypothetical mechanisms for simultaneous replication of the 5'-strand are presented as is the possibility that the tertiary structure of the DNA, as for example, a circular form of the helix, is of prime importance in in vivo replication.

MeSH Terms
Bacteriophages DNA/biosynthesis Deoxyribonucleases Escherichia coli/enzymology Nucleotidyltransferases RNA, Messenger/biosynthesis
Chemicals
RNA, Messenger DNA Nucleotidyltransferases Deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mitra S
Kornberg A
References (20)
20 references, click to expand
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.49.6.59
Published
1966-07-00
Pages
59-79
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2195533
Subset
IM
External Links
PubMed source DOI: 10.1085/jgp.49.6.59 PMC Full Text View journal details
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