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PMID: 5338876 Published · ppublish English Journal Article

Ribonucleic acid synthesis by Escherichia coli C 3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties of the coliphage-induced double-stranged ribonucleic acid.

The Biochemical journal ·Vol. 100 ·No. 3 ·1966-09-00 ·Pages 601-13

Bishop DH

Abstract

1. The efficiency of extracting nucleic acids from Escherichia coli after five methods of obtaining cell lysis was determined. 2. The recovery of various nucleic acid species isolated after chromatography on methylated albumin-coated kieselguhr was also examined. 3. Double-stranded coliphage-induced RNA was isolated from infected bacteria and its resistance to ribonuclease digestion under various conditions determined. 4. The involvement of double-stranded RNA during the infection process was demonstrated. 5. The time-course of the syntheses in infected cells of double-stranded RNA, DNA, single-stranded coliphage and 16s ribosomal RNA, transfer RNA and ribosomal 23s RNA was examined. 6. It was demonstrated that the syntheses of DNA, transfer RNA and ribosomal RNA decreased 10-15min. after infection. 7. Synthesis of coliphage RNA commenced 10-15min. after infection and double-stranded RNA was also synthesized from about 10min. after coliphage adsorption.

MeSH Terms
Bacteriolysis Carbon Isotopes Chromatography Coliphages/metabolism DNA, Bacterial/biosynthesis Escherichia coli/metabolism Nucleic Acids/analysis Phosphates/metabolism Phosphorus Isotopes RNA, Bacterial/biosynthesis RNA, Transfer/biosynthesis RNA, Viral/biosynthesis Thymine/metabolism Uracil/metabolism
Chemicals
Carbon Isotopes DNA, Bacterial Nucleic Acids Phosphates Phosphorus Isotopes RNA, Bacterial RNA, Viral Uracil RNA, Transfer Thymine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Bishop D H
References (22)
22 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1000601
Published
1966-09-00
Pages
601-13
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1265191
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1000601 PMC Full Text View journal details
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