An improved method for the rapid isolation of brain ribonucleic acid.

The Biochemical journal ·Vol. 183 ·No. 1 ·1979-10-01 ·Pages 181-4

Kaplan BB, Bernstein SL, Gioio AE

Abstract

An improved one-step method for the extraction of RNA from rat brain is described. Fresh or frozen tissue is disrupted in the powerful protein denaturant guanidine thiocyanate, and RNA isolated by ultracentrifugation through CsCl. The procedure is advantageous in that it is relatively simple, is rapid and does not expose the sample to enzyme treatments or repeated organic extractions.

MeSH Terms

Animals Brain Chemistry Centrifugation, Density Gradient/methods Cesium Male RNA/isolation & purification Rats

Chemicals

Cesium RNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kaplan B B

Bernstein S L

Gioio A E

References (17)

17 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1830181

Published

1979-10-01

Pages

181-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1161490

Subset

External Links

PubMed source DOI: 10.1042/bj1830181 PMC Full Text View journal details

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An improved method for the rapid isolation of brain ribonucleic acid.

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