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PMID: 534529 Published · ppublish English Journal Article

Translational activity of messenger ribonucleic acid isolated from unstimulated and phytohaemagglutinin-activated lymphocytes.

The Biochemical journal ·Vol. 184 ·No. 2 ·1979-11-15 ·Pages 277-82

Wallace DM, Jagus R, Benzie CR, Kay JE

Abstract

Purified cytoplasmic poly(A)+ RNA isolated from unstimulated pig lymphocytes has the same ability to direct translation in a range of cell-free systems as the corresponding mRNA from 20h phytohaemagglutinin-activated lymphocytes. Additional methylation of the mRNA is not required for maximum protein synthesis in the wheat-germ cell-free system. Misleading results are obtained if the mRNA preparations used are not adequately purified, and a method suitable for routine assessment of the degree of purification achieved is described. Cell-free protein-synthesizing systems from unstimulated lymphocytes translate added lymphocyte mRNA with lower efficiency than do comparable systems from phytohaemagglutinin-activated lymphocytes, whatever the source of the mRNA used.

MeSH Terms
Animals Cell-Free System Cytoplasm/analysis Lymphocyte Activation Lymphocytes/analysis Peptide Chain Initiation, Translational Phytohemagglutinins/pharmacology Poly A Protein Biosynthesis RNA, Messenger/blood,genetics,isolation & purification Swine
Chemicals
Phytohemagglutinins RNA, Messenger Poly A
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wallace D M
Jagus R
Benzie C R
Kay J E
References (28)
28 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1840277
Published
1979-11-15
Pages
277-82
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1161762
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1840277 PMC Full Text View journal details
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