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PMID: 535497 Published · ppublish English Journal Article

An analysis of spindle ultrastructure during prometaphase and metaphase of micronuclear division in Tetrahymena.

Chromosoma ·Vol. 75 ·No. 3 ·1979-00-00 ·Pages 293-308

LaFountain JR, Davidson LA

Abstract

Mitotic micronuclei were isolated from Tetrahymena thermophila in a medium containing hexylene glycol and their ultrastructure was analyzed using thin section techniques. The two stages selected for analysis were early prometaphase and metaphase. A comparison of data from these two stages revealed several differences in nuclear morphology. Metaphase nuclei were longer, they contained more microtubules, and the distribution of microtubules at metaphase was different from that at early prometaphase. Increases in microtubules, which are a unique class of microtubules that can be distinguished from other classes on the basis of their close association to the nuclear membrane. Growth of peripheral sheath microtubules is thought to be significant because it could be the mechanical basis of nuclear elongation. Crossbridges were observed throughout the spindle between all classes of microtubules, but the exact function of these elements remains to be determined.

MeSH Terms
Animals Cell Cycle Cell Nucleus/ultrastructure Interphase Metaphase Microscopy, Electron Microtubules/ultrastructure Tetrahymena/ultrastructure
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
LaFountain J R
Davidson L A
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24 references, click to expand
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00293473
Published
1979-00-00
Pages
293-308
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00293473 View journal details
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