Home LiteratureArticle Details
PMID: 5359607 Published · ppublish English Journal Article

Characteristics of virulent, attenuated, and avirulent Mycoplasma pneumoniae strains.

Journal of bacteriology ·Vol. 100 ·No. 2 ·1969-11-00 ·Pages 1037-43

Lipman RP, Clyde WA, Denny FW

Abstract

Homologous pairs of virulent and attenuated or avirulent Mycoplasma pneumoniae strains were derived and compared in an effort to elucidate the mechanisms of virulence. These related strains were found to vary in growth, glycolysis, protein electrophoretic patterns, peroxide formation, morphology, and cytadsorption. Variations in the last two characteristics closely correlated with avirulence. This enables understanding of one stage in the pathogenic sequence and provides a convenient marker for avirulence. The derivation of infectious avirulent strains may make possible their use as live vaccines against M. pneumoniae disease.

MeSH Terms
Animals Bacterial Proteins/analysis Chick Embryo Complement Fixation Tests Cricetinae Culture Media Electrophoresis Microscopy, Electron Mycoplasma/analysis,classification,cytology,growth & development,immunology,pathogenicity Mycoplasma Infections/therapy Vaccines Virulence
Chemicals
Bacterial Proteins Culture Media Vaccines
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lipman R P
Clyde W A
Denny F W
References (24)
24 references, click to expand
  1. Hemolysis in identifying Eaton's pleuro-pneumonia-like organism.
    Science. 1963 Jan 4;139(3549):55 PMID: 14021799
  2. MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA.
    J Immunol. 1964 Jun;92:958-65 PMID: 14218303
  3. Factors influencing growth inhibition of Mycoplasma pneumoniae by immune sera.
    Proc Soc Exp Biol Med. 1967 Oct;126(1):161-6 PMID: 6066157
  4. Growth inhibition test for identification of Mycoplasma species utilizing dried antiserum-impregnated paper discs.
    J Bacteriol. 1967 Apr;93(4):1392-6 PMID: 5340308
  5. Mycoplasma infections of man.
    N Engl J Med. 1965 Dec 2;273(23):1257-64 concl PMID: 5320783
  6. The interrelationship of virulence, cytadsorption, and peroxide formation in Mycoplasma pneumoniae.
    Proc Soc Exp Biol Med. 1969 Sep;131(4):1163-7 PMID: 5811969
  7. Adsorption of Mycoplasma pneumoniae to neuraminic acid receptors of various cells and possible role in virulence.
    J Bacteriol. 1968 Sep;96(3):695-705 PMID: 4183967
  8. Hemolysin of Mycoplasma pneumoniae: tentative identification as a peroxide.
    Science. 1965 Oct 8;150(3693):226-8 PMID: 5891190
  9. Application of a microtechnique to viral serological investigations.
    J Immunol. 1962 Mar;88:320-9 PMID: 13910995
  10. Temperature-sensitive mutants of Mycoplasma pneumoniae. I. In vitro biologic properties.
    J Infect Dis. 1969 Aug;120(2):217-24 PMID: 4898043
  11. Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum.
    J Exp Med. 1950 Nov 1;92(5):441-62 PMID: 14778924
  12. EXPERIMENTAL INFECTION WITH MYCOPLASMA PNEUMONIAE (EATON'S AGENT).
    J Exp Med. 1965 Jun 1;121:1071-86 PMID: 14319403
  13. Immunologic aspects of experimental Mycoplasma pneumoniae infection.
    J Infect Dis. 1969 Mar;119(3):255-66 PMID: 4181003
  14. Prevention of disease due to Mycoplasma pneumoniae.
    J Infect Dis. 1969 Aug;120(2):255-7 PMID: 5821108
  15. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO.
    Proc Natl Acad Sci U S A. 1962 Jan 15;48:41-9 PMID: 13878126
  16. INFECTION WITH ARTIFICIALLY PROPAGATED EATON AGENT (MYCOPLASMA PNEUMONIAE). IMPLICATIONS FOR DEVELOPMENT OF ATTENUATED VACCINE FOR COLD AGGLUTININ-POSITIVE PNEUMONIA.
    JAMA. 1964 Feb 8;187:442-7 PMID: 14079718
  17. Inactivated Mycoplasma pneumoniae vaccine. Evaluation in volunteers.
    JAMA. 1967 Feb 6;199(6):353-8 PMID: 5334595
  18. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  19. Studies on the electron transfer system. LXVII. Polyacrylamide gel electrophoresis of the mitochondrial electron transfer complexes.
    Arch Biochem Biophys. 1966 Apr;114(1):223-30 PMID: 4289034
  20. Physical properties of human Mycoplasma species.
    J Bacteriol. 1966 Jul;92(1):214-9 PMID: 5941277
  21. Studies on sensitivity to streptomycin of the atypical pneumonia agent.
    J Bacteriol. 1957 Dec;74(6):784-7 PMID: 13502305
  22. Protective effects of inactive Mycoplasma pneumoniae vaccine in military personnel, 1964-1966.
    Am Rev Respir Dis. 1968 Mar;97(3):359-65 PMID: 4867003
  23. EATON PLEUROPNEUMONIA-LIKE ORGANISM (MYCOPLASMA PNEUMONIAE) COMPLEMENT-FIXING ANTIGEN: EXTRACTION WITH ORGANIC SOLVENTS.
    J Immunol. 1965 Jul;95:19-25 PMID: 14322576
  24. Identification of Mycoplasma and other microorganisms by polyacrylamide-gel electrophoresis of cell proteins.
    J Bacteriol. 1967 Dec;94(6):1807-10 PMID: 4864403
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.100.2.1037-1043.1969
Published
1969-11-00
Pages
1037-43
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC250191
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.100.2.1037-1043.1969 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2