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PMID: 5359609 Published · ppublish English Journal Article

Ultrastructural changes in bacteria isolated from cases of leprosy.

Journal of bacteriology ·Vol. 100 ·No. 2 ·1969-11-00 ·Pages 1062-75

Freer J, Kim KS, Krauss MR, Beaman L, Barksdale L

Abstract

One of two groups of rod-shaped bacteria (bacilli) isolated from cases of human leprosy have been found, under certain cultural conditions, to give rise to unusual phenotypes. In electron micrographs of ultrathin sections and negatively stained whole cells of these osmotically fragile bacteria, ultrastructural anomalies are apparent and seem to arise from disorder in the process(es) of septum formation or cell envelope biosynthesis or both. Two of the four strains examined are related to Corynebacterium acnes.

MeSH Terms
Corynebacterium/cytology,isolation & purification Culture Media Humans Leprosy/microbiology Methods Microscopy, Electron Osmotic Fragility Phenotype
Chemicals
Culture Media
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Freer J
Kim K S
Krauss M R
Beaman L
Barksdale L
References (6)
6 references, click to expand
  1. Approaches to cultivation of M. leprae in a new laboratory.
    Int J Lepr. 1965 Jul-Sep;33(3):Suppl:527-32 PMID: 5322701
  2. Phage-host relationships in nontoxigenic and toxigenic diphtheria bacilli.
    J Bacteriol. 1954 Feb;67(2):220-32 PMID: 13129217
  3. Araldite as an embedding medium for electron microscopy.
    J Biophys Biochem Cytol. 1958 Mar 25;4(2):191-4 PMID: 13525433
  4. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states.
    J Biophys Biochem Cytol. 1958 Nov 25;4(6):671-8 PMID: 13610928
  5. ALDEHYDE FIXATION FOR MORPHOLOGICAL AND ENZYME HISTOCHEMICAL STUDIES WITH THE ELECTRON MICROSCOPE.
    J Histochem Cytochem. 1964 Feb;12:57-71 PMID: 14187313
  6. A ROUTINE TECHNIQUE FOR DOUBLE-STAINING ULTRATHIN SECTIONS USING URANYL AND LEAD SALTS.
    J Cell Biol. 1965 Apr;25:157-61 PMID: 14289358
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1969-11-00
Pages
1062-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC250194
Subset
IM
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