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PMID: 537913 Published · ppublish English Comparative Study Journal Article

Cloning and characterization of ribosomal RNA genes from wheat and barley.

Nucleic acids research ·Vol. 7 ·No. 7 ·1979-12-11 ·Pages 1869-85

Gerlach WL, Bedbrook JR

Abstract

Wheat and barley DNA enriched for ribosomal RNA genes was isolated from actinomycin D-CsCl gradients and used to clone the ribosomal repeating units in the plasmid pAC184. All five chimeric plasmids isolated which contained wheat rDNA and eleven of the thirteen which had barley rDNA were stable and included full length ribosomal repeating units. Physical maps of all length variants cloned have been constructed using the restriction endonucleases Eco Rl, Bam Hl, Bgl II, Hind III and Sal I. Length variation in the repeat units was attributed to differences in the spacer regions. Comparison of Hae III and Hpa II digestion of cereal rDNAs and the cloned repeats suggests that most methylated cytosines in natural rDNA are in -CpG-. Incomplete methylation occurs at specific Bam Hl sites in barley DNA. Detectable quantities of ribosomal spacer sequences are not present at any genomic locations other than those of the ribosomal RNA gene repeats.

MeSH Terms
Base Sequence Chromosome Mapping Cloning, Molecular Cytosine DNA, Recombinant Edible Grain/genetics Genes Hordeum/genetics Methylation Plasmids RNA, Ribosomal/genetics Triticum/genetics
Chemicals
DNA, Recombinant RNA, Ribosomal Cytosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gerlach W L
Bedbrook J R
References (21)
21 references, click to expand
  1. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  2. Characterization of a cloned ribosomal fragment from mouse which contains the 18S coding region and adjacent spacer sequences.
    Nucleic Acids Res. 1979 Apr;6(4):1351-69 PMID: 377227
  3. Absence of cytosine methylation at C-C-G-G and G-C-G-C sites in the rDNA coding regions and intervening sequences of Drosophila and the rDNA of other insects.
    Nucleic Acids Res. 1979 Jul 11;6(9):2987-95 PMID: 493131
  4. Specificity of Hpa II and Hae III DNA methylases.
    Nucleic Acids Res. 1977 Dec;4(12):4211-21 PMID: 600794
  5. Use of restriction enzymes to study eukaryotic DNA methylation: I. The methylation pattern in ribosomal DNA from Xenopus laevis.
    J Mol Biol. 1978 Jan 5;118(1):27-47 PMID: 625056
  6. A general method for the purification of restriction enzymes.
    Nucleic Acids Res. 1978 Jul;5(7):2373-80 PMID: 673857
  7. Sequence specificity of DNA methylases from Bacillus amyloliquefaciens and Bacillus brevis.
    J Mol Biol. 1978 Oct 5;124(4):701-11 PMID: 712853
  8. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
    Cell. 1976 Nov;9(3):431-8 PMID: 1033038
  9. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
    Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
  10. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  11. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  12. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  13. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
    J Bacteriol. 1972 May;110(2):667-76 PMID: 4336693
  14. Ribosomal RNA cistron multiplicity and nucleolar organizers in hexaploid wheat.
    Genetics. 1974 Jan;76(1):33-44 PMID: 4361912
  15. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.
    Biochemistry. 1973 Dec 4;12(25):5045-50 PMID: 4366077
  16. The role of homoeologous group 1 chromosomes in the control of rRNA genes in wheat.
    Biochem Genet. 1974 Oct;12(4):271-9 PMID: 4441362
  17. Separation and analysis of promoter sites in bacteriophage lambda DNA by specific endonucleases.
    J Mol Biol. 1974 Jan 5;85(4):475-84 PMID: 4546908
  18. Ribosomal RNA genes and plant development.
    Nature. 1972 Jan 7;235(5332):30-2 PMID: 4550391
  19. Kinetic complexity of RNA molecules.
    J Mol Biol. 1972 Jan 14;63(1):21-39 PMID: 4622766
  20. Endonuclease recognition sites mapped on Zea mays chloroplast DNA.
    Proc Natl Acad Sci U S A. 1976 Dec;73(12):4309-13 PMID: 16592373
  21. The genes for cytoplasmic ribosomal ribonucleic Acid in higher plants.
    Plant Physiol. 1973 Apr;51(4):677-84 PMID: 16658392
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/7.7.1869
Published
1979-12-11
Pages
1869-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342353
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/7.7.1869 PMC Full Text View journal details
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